Recombinant Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1), partial

Code CSB-YP614387HU
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Source Yeast
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Code CSB-EP614387HU
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Source E.coli
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Code CSB-EP614387HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614387HU
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Source Baculovirus
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Code CSB-MP614387HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIAM1
Uniprot No.
Alternative Names
Human T-lymphoma invasion and metastasis inducing TIAM1 protein; T cell lymphoma invasion and metastasis 1; T lymphoma invasion and metastasis inducing TIAM1 protein; T-lymphoma invasion and metastasis-inducing protein 1; TIAM-1; TIAM1; TIAM1_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration.
Gene References into Functions
  1. Study in hepatocellular carcinoma (HCC) cells proves that SETDB1 promotes the proliferation and migration of cells by forming SETDB1-Tiam1 compounds. SETDB1-Tiam1 compounds were involved in a novel pathway, which regulated epigenetic modification of gene expression in HCC patients samples. PMID: 29739365
  2. Down-regulating Tiam1 expression resulted in the down-regulation of circRNA-ACAP2 expression and up-regulation of miR-21-5P expression. PMID: 30212824
  3. The effects of Tiam1 on metastasis and EMT mediated by the Wnt/beta-catenin pathway were reversed by Rac1 silencing, suggesting that the prometastatic effect of Tiam1 is mediated by the activation of Rac1. These results indicate that Tiam1 may be a prognostic factor and potential therapeutic target for the treatment of thyroid cancers. PMID: 29277502
  4. These findings highlight a regulatory pathway of Tiam1/Rac1 in Th17 cells and suggest that it may be a therapeutic target in multiple sclerosis. PMID: 27725632
  5. Competing interactions of EndoA3 with membrane versus TIAM1 modulate hyperproliferative and metastatic phenotypes. Disruption of EndoA3-membrane interactions stimulates TIAM1 and small GTPases in vitro, and further promotes pro-metastatic phenotypes in vivo. PMID: 29920278
  6. PDZ domains direct protein-protein interactions and serve as models for protein design. This study optimized a protein design energy function for the Tiam1 and Cask PDZ domains that combines a molecular mechanics energy, Generalized Born solvent, and an empirical unfolded state model. PMID: 28394603
  7. Clinical outcome was improved in tumors where a TIAM1 variant was present concomitantly with either ALK mutation or MYCN amplification. PMID: 28423360
  8. the Ser179Glu mutant of SDC-4 binds strongly Tiam1, a Rac1-GEF reducing Rac1-GTP by 3-fold in MCF-7 breast adenocarcinoma cells. PMID: 29121646
  9. results highlight the role of the PHn-CC-Ex domain in Tiam1 GEF regulation and suggest a combinatorial model for GEF inhibition and activation of the Rac1 signaling pathway. PMID: 28882897
  10. Tiam1 expression is frequently up-regulated in breast cancer. Tiam1 expression correlated with clinicopathological parameters. PMID: 27562113
  11. In colorectal carcinoma TIAM1 suppresses tumor progression by regulating YAP/TAZ activity. PMID: 28416184
  12. The expression of TIAM1 is closely related to the occurrence, development and metastasis of oral squamous cell carcinoma , and it can be used as a new marker for reflecting its biological behaviors. PMID: 28685528
  13. TIAM1 was aberrantly expressed in the oral squamous cell carcinoma group compared with the normal group. Furthermore, high expression of TIAM1 was significantly correlated with lymph node metastasis and the timing of recurrence. PMID: 27862620
  14. These data suggest a novel link between Tiam1 and RhoG/ILK /ELMO2 pathway as upstream effectors of the Rac1-mediated phagocytic process in trabecular meshwork cells. PMID: 27539661
  15. we have identified a novel reverse signaling pathway acting through Tiam1 and Rac that promotes aggressive behavior in OSCC expressing S4D and PB1. PMID: 28038319
  16. Taken together, these results suggest that the Par3 regulates invasion and metastasis in pancreatic cancers by controlling tight junction assembly via Tiam1. PMID: 26084985
  17. Tiam1 expression significantly increased in cholangiocarcinoma tissues, and increased along with the degree of malignancy of cholangiocarcinoma. Targeted silencing Tiam1 expression could inhibit proliferation and migration activity of cholangiocarcinoma cells. PMID: 26884821
  18. We identified T lymphoma invasion and metastasis 1 (TIAM1) gene as potential target of miR-329 in gastric cancer PMID: 25654811
  19. Heterodimeric CUL3 proteinubiquitylates TIAM1.CUL3 regulates TIAM1 abundance and subsequent RAC1 signaling. PMID: 25684205
  20. The downregulation of MiR-182 is associated with the growth and invasion of osteosarcoma cells through the regulation of TIAM1 expression. PMID: 25973950
  21. Study identifies a bidirectional (phosphorylation and dephosphorylation) regulatory mechanism controlling Tiam1 stability and provides new insights on how EGFR signaling triggers Rac1 activation and cancer development. PMID: 25746002
  22. The polymorphisms rs2833188 and rs2833195 in TIAM1 gene may be associated with some clinical characteristics in children with Kawasaki disease. PMID: 26575881
  23. These results suggested that Tiam1 overexpression in LSCC is possibly involved in the promotion of migration and invasion, and is a promising therapeutic target in the prevention of the progression of LSCC. PMID: 25672412
  24. we show that HUWE1 stimulates human lung cancer cell invasion through regulating TIAM1 stability. Finally, we demonstrate that HUWE1 and TIAM1 protein levels are inversely correlated in human lung carcinomas PMID: 25543140
  25. TIAM1 is activated by phosphatidylinositol 5-phosphate which activates RAC1, regulating actin dynamics and cell invasion. PMID: 24905281
  26. MiR-29b suppresses tumor growth and metastasis in colorectal cancer via downregulating Tiam1 expression. PMID: 25032858
  27. These data demonstrate, that in addition to microtubule-independent Tiam1 activation, HGF engages additional microtubule- and APC-dependent pathway of Asef activation. PMID: 25101856
  28. Authors propose a model for Rac1 activation where SIRT1/2 positively modulates the DVL/TIAM1/Rac1 axis and promotes sustained pathway activation. PMID: 24362520
  29. Data support the notion that Tiam1 plays an important role in the progression of nasopharyngeal carcinoma (NPC), and its overexpression is associated with malignant phenotypes of NPC. PMID: 24912585
  30. The rs13048019 in the TIMA1 gene are unlikely to be common cause of SALS in Chinese population PMID: 24493373
  31. Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033
  32. TIAM1-RAC1-NOX2 signalling axis is activated in the initial stages of diabetes to increase intracellular ROS leading to mitochondrial damage and accelerated capillary cell apoptosis PMID: 24554007
  33. miR-141 functions as a tumor suppressor and inhibits the migration and invasion of hepatocellular carcinoma cells by targeting Tiam1. PMID: 24551096
  34. Our results uncover that inhibition of proteasome-mediated Tiam1 degradation is an upstream event leading to Rac1/JNK activation and cell apoptosis in response to DNA-damaging drug treatment. PMID: 24737324
  35. Tiam1 expression may be beneficial in predicting lymph node metastasis and survival of patients with lung adenocarcinoma PMID: 24661909
  36. Data show that T lymphoma invasion and metastasis protein (Tiam1) modulates the cell invasion, mediated by actin cytoskeleton remodeling in retinoblastoma (RB). PMID: 23950931
  37. nitric oxide is a key molecule promoting endothelial cell permeability, linking the cytoskeleton to VE-cadherin phosphorylation through regulation of the endothelial-junction-associated Rac guanine-nucleotide-exchange factor (GEF) TIAM1. PMID: 24046447
  38. Tiam1 associates with EphA2 receptors in early endosomes and is required for endocytic trafficking of EphA2 receptors. PMID: 24112471
  39. Due to their specificity over related GEF proteins they may represent promising tools for further elucidation of the biological functions of Tiam1. PMID: 23757206
  40. Tiam1 contribute to chronic lymphocytic leukemia cells proliferation and chemoresistance. PMID: 24501217
  41. The 5-LOX/LTC4 /CysLT1 signaling pathway regulates EGF-induced cell migration by increasing Tiam1 expression. PMID: 24350867
  42. MiR-22 expression inhibited TIAM1 mRNA and protein expression. PMID: 23440286
  43. Data show that T-lymphoma invasion and metastasis inducing factor 1 (Tiam1) may contribute to squamous-cell carcinoma of the head and neck (SCCH) progression, and represent as a prognostic indicator as well as a potential therapeutic target for SCCHN. PMID: 24189000
  44. Downregulation of Tcell lymphoma invasion and metastasisinducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells. PMID: 23754572
  45. In gastric cancer cells, Tiam1 affects multiple properties associated with acquisition of the metastatic phenotype, and may be a marker of gastric cancer progression and metastasis in a subset of cancer. PMID: 23422181
  46. Over-expression of Tiam-1 contributes to the invasive phenotype of gastric cancer cells. PMID: 23534724
  47. these results suggest that the progression of pancreatic tumors is partially controlled by the balance between Tiam1-rac1 and RhoA. PMID: 23322732
  48. Tiam1 is closely related with invasion and metastasis of breast carcinoma. PMID: 23291131
  49. Tiam1 and Rac1 expressions may be independent predictors for the poor prognosis in patients with primary gallbladder carcinoma PMID: 21853342
  50. Tiam1 is an invasion and metastasis inducing factor of lung carcinoma. The overexpression of Tiam1 is closely associated with lymph node metastases, TNM stage and histological types of lung carcinoma. PMID: 22027789

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Subcellular Location
Cell junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
TIAM family
Tissue Specificity
Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.
Database Links

HGNC: 11805

OMIM: 600687

KEGG: hsa:7074

STRING: 9606.ENSP00000286827

UniGene: Hs.517228

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