Recombinant Human TBC1 domain family member 20(TBC1D20),partial

Code CSB-YP846594HU1
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Source Yeast
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Code CSB-EP846594HU1
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Source E.coli
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Code CSB-EP846594HU1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846594HU1
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Source Baculovirus
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Code CSB-MP846594HU1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names TBC1D20
Uniprot No. Q96BZ9
Alternative Names TBC1D20; C20orf140; TBC1 domain family member 20
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
GTPase-activating protein specific for Rab1 and Rab2 small GTPase families for which it can accelerate the intrinsic GTP hydrolysis rate by more than five orders of magnitude.
Gene References into Functions
  1. Warburg Micro syndrome is caused by TBC1D20 deficiency. PMID: 26063829
  2. Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans. PMID: 24239381
  3. The NS5A interaction with TBC1D20 and Rab1 is essential for the viral life cycle. PMID: 22491470
  4. These findings add TBC1D20 to the network of host factors regulating HIV replication cycle. PMID: 22260459
  5. TBC1D20 was found to be the first known GAP for Rab1, which is implicated in the regulation of anterograde traffic between the endoplasmic reticulum and the Golgi complex PMID: 17901050

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Involvement in disease Warburg micro syndrome 4 (WARBM4)
Subcellular Location Membrane; Multi-pass membrane protein.
Database Links

HGNC: 16133

OMIM: 611663

KEGG: hsa:128637

STRING: 9606.ENSP00000346139

UniGene: Hs.590876

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7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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