Recombinant Human Tescalcin (TESC)

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Code CSB-EP023393HU
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Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
TESC
Uniprot No.
Research Area
Signal Transduction
Alternative Names
TESC; CHP3Calcineurin B homologous protein 3; Tescalcin; TSC
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-214aa
Target Protein Sequence
GAAHSASEEVRELEGKTGFSSDQIEQLHRRFKQLSGDQPTIRKENFNNVPDLELNPIRSKIVRAFFDNRNLRKGPSGLADEINFEDFLTIMSYFRPIDTTMDEEQVELSRKEKLRFLFHMYDSDSDGRITLEEYRNVVEELLSGNPHIEKESARSIADGAMMEAASVCMGQMEPDQVYEGITFEDFLKIWQGIDIETKMHVRFLNMETMALCH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
40.6kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Promotes the maturation, transport, cell surface stability and exchange activity of SLC9A1/NHE1 at the plasma membrane. Promotes the induction of hematopoietic stem cell differentiation toward megakaryocytic lineage. Essential for the coupling of ERK cascade activation with the expression of ETS family genes in megakaryocytic differentiation. Also involved in granulocytic differentiation in a ERK-dependent manner. Inhibits the phosphatase activity of calcineurin.
Gene References into Functions
  1. This study found that the genotype of TESC at the rs7294919 locus and MDD had a significant interactive effect on the hippocampal subfield volume. PMID: 28575645
  2. High Tescalcin expression is associated with colorectal cancer. PMID: 27485112
  3. CHP3 functions as a novel negative regulator of cardiomyocyte hypertrophy via inhibition of GSK3alpha/beta phosphorylation. PMID: 25935310
  4. This study demonstrated that highly significant effects of rs7294919 of tescalcin on hippocampal gray matter volumes. PMID: 24776739
  5. a binding partner and regulator of subunit 4 of the COP9 signalosome PMID: 24659803
  6. TESC is overexpressed in colorectal cancer PMID: 24811141
  7. Loss of both N-myristoylation and calcium binding of CHP3 decreases cell surface stability of NHE1. PMID: 22984264
  8. Tescalcin is one of the key gene products that is involved in switching differentiation program in some cell types. PMID: 20060826
  9. CHP3 binds directly to the proximal juxtamembrane C-terminal region (amino acids 505-571) of rat NHE1 in the same region that binds CHP1 and CHP2. PMID: 18321853

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Subcellular Location
Nucleus. Cytoplasm. Membrane; Lipid-anchor. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle membrane.
Protein Families
Calcineurin regulatory subunit family, CHP subfamily
Tissue Specificity
Expressed in mature megakaryocytes and polymorphonuclear granulocytes (at protein level). Abundantly expressed in heart. Also expressed at a lower level in adult testis and salivary gland, and in the placenta.
Database Links

HGNC: 26065

OMIM: 611585

KEGG: hsa:54997

STRING: 9606.ENSP00000334785

UniGene: Hs.525709

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