Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TMX2

Uniprot No.

Q9Y320

Research Area

Cell Biology

Alternative Names

Cell proliferation inducing gene 26 protein; Cell proliferation-inducing gene 26 protein; CGI 31; Growth inhibiting gene 11; PDIA12; PIG26; Protein disulfide isomerase family A; member 12; Thioredoxin domain containing protein 14; Thioredoxin domain-containing protein 14; Thioredoxin related transmembrane protein 2; Thioredoxin-related transmembrane protein 2; tmx2; TMX2_HUMAN; TXNDC14

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

49-102aa

Target Protein Sequence

LPTQREDGNPCDFDWREVEILMFLSAIVMMKNRRSITVEQHIGNIFMFSKVANT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.3kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 49-102 form the expressed segment for recombinant Human TMX2. The calculated molecular weight for this TMX2 protein is 33.3 kDa. The TMX2 protein was expressed in e.coli. The TMX2 coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant TMX2 protein in following stages of expression and purification.

Thioredoxin-related transmembrane protein 2 (TMX2) is a member of the thioredoxin superfamily, playing a role in redox regulation within the endoplasmic reticulum (ER). TMX2 contains a thioredoxin-like domain and multiple transmembrane helices, suggesting its association with the ER membrane. As part of the ER redox system, TMX2 is involved in maintaining protein folding and quality control by participating in disulfide bond formation and reduction. It may interact with other ER-resident proteins and contribute to the regulation of cellular responses to oxidative stress. Research on TMX2 spans various areas, including investigations into its specific redox functions, potential interactions with client proteins, and implications in cellular processes related to ER homeostasis.

Target Background

Function

Endoplasmic reticulum and mitochondria-associated protein that probably functions as a regulator of cellular redox state and thereby regulates protein post-translational modification, protein folding and mitochondrial activity. Indirectly regulates neuronal proliferation, migration, and organization in the developing brain.

Gene References into Functions

TMX2 is enriched on the mitochondria-associated membrane. Targeting TMX to the MAM requires palmitoylation of two membrane-proximal cytosolic cysteines. PMID: 22045338
molecular cloning and characterization of one member of the thioredoxin superfamily, designated as TMX2; the TMX2 cDNA consists of 1644 nucleotides and contains an open reading frame encoding a protein of 372 amino acids PMID: 12670024

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Mitochondrion membrane.

Tissue Specificity

Widely expressed.

Database Links

HGNC: 30739

OMIM: 616715

KEGG: hsa:51075

STRING: 9606.ENSP00000278422

UniGene: Hs.654965

Code	CSB-EP023951HU
Abbreviation	Recombinant Human TMX2 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Thioredoxin-related transmembrane protein 2 (TMX2), partial

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