Recombinant Human Transaldolase (TALDO1), partial

Code CSB-RP042754h
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
TALDO1
Uniprot No.
Research Area
Metabolism
Alternative Names
Dihydroxyacetone transferase; EC 2.2.1.2; EPS8L2 ; Glycerone transferase ; TAL; TAL H; TALDO; TALDO_HUMAN; TALDO1; TALDOR ; TALH ; Transaldolase 1; Transaldolase
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
9-337aa
Target Protein Sequence
QRMESALDQLKQFTTVVADTGDFHAIDEYKPQDATTNPSLILAAAQMPAYQELVEEAIAYGRKLGGSQEDQIKNAIDKLFVLFGAEILKKIPGRVSTEVDARLSFDKDAMVARARRLIELYKEAGISKDRILIKLSSTWEGIQAGKELEEQHGIHCNMTLLFSFAQAVACAEAGVTLISPFVGRILDWHVANTDKKSYEPLEDPGVKSVTKIYNYYKKFSYKTIVMGASFRNTGEIKALAGCDFLTISPKLLGELLQDNAKLVPVLSAKAAQASDLEKIHLDEKSFRWLHNEDQMAVEKLSDGIRKFAADAVKLERMLTERMFNAENGK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
63.7kDa
Protein Length
Partial
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.
Gene References into Functions
  1. These results demonstrate that the nucleocytoplasmic distribution of TALDO1, modulated via alternative translational initiation and dimer formation, plays an important role in a wide range of metabolic networks PMID: 27703206
  2. The above findings support the premise that biallelic mutations in TALDO1 are responsible for transaldolase deficiency and confirm the broad phenotypic variability of this condition, even with the same genotype. PMID: 25388407
  3. This study expands the clinical definition of transaldolase deficiency, and adds to its allelic heterogeneity. PMID: 23315216
  4. Data suggest that exchange reactions during gluconeogenesis catalyzed by transaldolase and triose-phosphate isomerase do not differ between subjects with type 2 diabetes and control subjects under fasting or hyperglycemic conditions. PMID: 23736541
  5. renal phenotype of patients with transaldolase deficiency PMID: 22510381
  6. these data provide strong experimental evidence that transaldolase exchange occurs in humans, resulting in an overestimate of gluconeogenesis PMID: 21062960
  7. Data show that erythronic acid was identified as a major abnormal metabolite in all patients and in knock-out TALDO mice implicating an as yet unknown biochemical pathway in this disease. PMID: 20600873
  8. granzyme B-cleaved transaldolase-specific T cell-mediated cytotoxicity may contribute to the progressive destruction of oligodendrocytes in patients with multiple sclerosis PMID: 20194725
  9. transaldolase is regulated by ZNF143 in a tissue-specific manner PMID: 14702349
  10. TALase undergoes anterograde trafficking in neutrophils from nonpregnant individuals, whereas retrograde trafficking is found during pregnancy PMID: 16092052
  11. Mutation in the TALDO1 gene was found in patients with hydrops fetalis and neonatal multi-organ disease. PMID: 17095351
  12. A patch of functionally important amino acid residues extends from serine-171 toward the catalytic site and is proposed as a novel ligand shuttling path connecting these specific sites to the enzyme's active site. PMID: 17503352
  13. Transaldolase-deficient patients had significantly increased urinary heptoses, revealing novel urinary biomarkers for identification of the deficiency. PMID: 17603756
  14. A new case of TALDO deficiency resulted in cirrhosis, rickets and deafness. PMID: 18331807
  15. The present study identified the TAL deficiency as a modulator of mitochondrial homoeostasis, Ca(2+) fluxing and apoptosis. PMID: 18498245
  16. analysis of enzymes TalB and Taldo1 in human and E. coli PMID: 18687684
  17. genetic polymorphisms in Transaldolase 1 are associated with squamous cell carcinoma of the head and neck . PMID: 18805652

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Involvement in disease
Transaldolase deficiency (TALDOD)
Subcellular Location
Cytoplasm.
Protein Families
Transaldolase family, Type 1 subfamily
Database Links

HGNC: 11559

OMIM: 602063

KEGG: hsa:6888

STRING: 9606.ENSP00000321259

UniGene: Hs.438678

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