Recombinant Human Transcription elongation factor SPT5 (SUPT5H), partial

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SUPT5H

Uniprot No.

Alternative Names

DRB sensitivity inducing factor large subunit; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; hSPT 5; hSPT5; SPT 5; SPT 5H; SPT5; SPT5H; SPT5H_HUMAN; Suppressor of Ty 5 homolog; Suppressor of Ty 5 homolog (S. cerevisiae); SUPT 5H; supt5h; Tat cotransactivator 1 protein; Tat CT1; Tat CT1 protein; Tat-cotransactivator 1 protein; Tat-CT1 protein; Transcription elongation factor SPT 5; Transcription elongation factor spt5; Transcription factor Tat CT1

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Gene References into Functions

These findings are consistent with a central role of Spt5 in maintenance of TFIID-promoter association and promoter escape to support rapid transcriptional induction and re-initiation of inflammatory-response genes. PMID: 27180651
The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. PMID: 28892040
OGA is physically associated with the known RNA polymerase II (pol II) pausing/elongation factors SPT5 and TRIM28-KAP1-TIF1beta, and a purified OGA-SPT5-TIF1beta complex has elongation properties. PMID: 27601472
A transcription-independent effect of tumor necrosis factor alpha on RNA splicing, mediated by Spt5. PMID: 26903558
SPT5 contributes to the up-regulation of hTERT expression and colonic tumor development. PMID: 26418880
Data indicate that the Plus3 domain of the Rtf1 subunit mediates Paf1C recruitment to genes by binding a repeating domain within the phosphorylated elongation factor Spt5. PMID: 24101474
DSIF Is Selectively Required for mRNA Splicing and Export of NF-kappaB Target Genes. PMID: 23041311
RNA polymerase II elongation repression is critically dependent on the C-terminus of Spt5 PMID: 19742326
phosphorylated by P-TEFb kinase during HIV-1 transcription in Tat/TAR dependent manner PMID: 15564463
hSpt5 function in transcription regulation and mRNA capping is essential for a subset of cellular and viral genes and may not be required for global gene expression. PMID: 15620346
evolutionarily conserved repetitive heptapeptide motif (consensus = G-S-R/Q-T-P) in the C-terminal region of hSpt5, like the C-terminal domain of RNA Pol II, is highly phosphorylated by P-TEFb PMID: 16427012
phosphorylation by cdk9; binds pin1 protein after phosphorylation PMID: 11575923

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Subcellular Location

Nucleus.

Protein Families

SPT5 family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 11469

OMIM: 602102

KEGG: hsa:6829

STRING: 9606.ENSP00000404029

UniGene: Hs.631604

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Recombinant Human Transcription elongation factor SPT5 (SUPT5H), partial

Product Details

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