Product Details

Uniprot No.

Target Names

SUPT5H

Alternative Names

DRB sensitivity inducing factor large subunit; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; hSPT 5; hSPT5; SPT 5; SPT 5H; SPT5; SPT5H; SPT5H_HUMAN; Suppressor of Ty 5 homolog; Suppressor of Ty 5 homolog (S. cerevisiae); SUPT 5H; supt5h; Tat cotransactivator 1 protein; Tat CT1; Tat CT1 protein; Tat-cotransactivator 1 protein; Tat-CT1 protein; Transcription elongation factor SPT 5; Transcription elongation factor spt5; Transcription factor Tat CT1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human SUPT5H protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21D1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SUPT5H, also known as SPT5, serves as the large subunit of the DRB sensitivity-inducing factor (DSIF) complex, a critical regulator of transcription elongation by RNA polymerase II. This protein plays dual roles in gene expression, functioning both as a negative elongation factor that induces promoter-proximal pausing and as a positive regulator that promotes productive elongation when phosphorylated. SUPT5H also participates in HIV-1 transcription as a Tat cotransactivator, making it relevant to both fundamental transcription biology and viral pathogenesis research.

This recombinant monoclonal antibody, generated from clone 21D1 in rabbit, offers the reproducibility and consistency that demanding experiments require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis of NIH/3T3 whole cell lysate, the antibody detects a band at approximately 150 kDa when used at 1:1000 dilution. This observed molecular weight exceeds the predicted 121 kDa, which is consistent with post-translational modifications such as phosphorylation or glycosylation that are known to affect SUPT5H migration. Immunohistochemistry validation in paraffin-embedded human colorectal cancer tissue at 1:100 dilution reveals clear nuclear staining patterns appropriate for this transcription factor. The antibody demonstrates cross-species reactivity with both human and mouse samples, supporting comparative studies across model systems.

This antibody serves researchers investigating transcription elongation mechanisms, chromatin dynamics, and gene regulation in both normal cellular processes and disease contexts including cancer and viral infection.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Gene References into Functions

These findings are consistent with a central role of Spt5 in maintenance of TFIID-promoter association and promoter escape to support rapid transcriptional induction and re-initiation of inflammatory-response genes. PMID: 27180651
The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. PMID: 28892040
OGA is physically associated with the known RNA polymerase II (pol II) pausing/elongation factors SPT5 and TRIM28-KAP1-TIF1beta, and a purified OGA-SPT5-TIF1beta complex has elongation properties. PMID: 27601472
A transcription-independent effect of tumor necrosis factor alpha on RNA splicing, mediated by Spt5. PMID: 26903558
SPT5 contributes to the up-regulation of hTERT expression and colonic tumor development. PMID: 26418880
Data indicate that the Plus3 domain of the Rtf1 subunit mediates Paf1C recruitment to genes by binding a repeating domain within the phosphorylated elongation factor Spt5. PMID: 24101474
DSIF Is Selectively Required for mRNA Splicing and Export of NF-kappaB Target Genes. PMID: 23041311
RNA polymerase II elongation repression is critically dependent on the C-terminus of Spt5 PMID: 19742326
phosphorylated by P-TEFb kinase during HIV-1 transcription in Tat/TAR dependent manner PMID: 15564463
hSpt5 function in transcription regulation and mRNA capping is essential for a subset of cellular and viral genes and may not be required for global gene expression. PMID: 15620346
evolutionarily conserved repetitive heptapeptide motif (consensus = G-S-R/Q-T-P) in the C-terminal region of hSpt5, like the C-terminal domain of RNA Pol II, is highly phosphorylated by P-TEFb PMID: 16427012
phosphorylation by cdk9; binds pin1 protein after phosphorylation PMID: 11575923

Hide All

Subcellular Location

Nucleus.

Protein Families

SPT5 family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 11469

OMIM: 602102

KEGG: hsa:6829

STRING: 9606.ENSP00000404029

UniGene: Hs.631604

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA269987A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:NIH/3T3 whole cell lysate(30µg) All lanes: SPT5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time：60s IHC image of CSB-RA269987A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Have Questions?	Leave a Message or Start an on-line Chat

SUPT5H Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background