Recombinant Human Transcription factor E2F5(E2F5)

Code CSB-YP624021HU
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Source Yeast
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Code CSB-EP624021HU
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Source E.coli
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Code CSB-EP624021HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP624021HU
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Source Baculovirus
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Code CSB-MP624021HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names E2F5
Uniprot No. Q15329
Alternative Names E2F 5; E2F transcription factor 5; E2F transcription factor 5 p130 binding; E2F-5; E2f5; E2F5_HUMAN; p130 binding; Transcription factor E2F5
Species Homo sapiens (Human)
Expression Region 1-346
Target Protein Sequence MAAAEPASSG QQAPAGQGQG QRPPPQPPQA QAPQPPPPPQ LGGAGGGSSR HEKSLGLLTT KFVSLLQEAK DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN SIQWKGVGAG CNTKEVIDRL RYLKAEIEDL ELKERELDQQ KLWLQQSIKN VMDDSINNRF SYVTHEDICN CFNGDTLLAI QAPSGTQLEV PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT QPSSQSLTPV TPQKSSMATQ NLPEQHVSER SQALQQTSAT DISSAGSISG DIIDELMSSD VFPLLRLSPT PADDYNFNLD DNEGVCDLFD VQILNY
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Gene References into Functions
  1. High E2F5 expression is associated with Glioma. PMID: 29362021
  2. knockdown of E2F5 and PFTK1 mimicked the tumor-suppressive effects of miR-1-3p overexpression on PCa progression. Conversely, concomitant knockdown of miR-1-3p and E2F5 and PFTK1 substantially reversed the inhibitory effects of either E2F5 or PFTK1 silencing alone. PMID: 30185212
  3. miR-1179 overexpression could also inhibit tumor growth in vivo by suppressing the expression of E2F5. PMID: 29859832
  4. FOXN3 functions as a tumor suppressor in hepatocellular carcinoma by downregulating the expression of E2F5. PMID: 27259277
  5. Taken together, these findings indicated that SNHG16 induces breast cancer cell migration by competitively binding miR-98 with E2F5. PMID: 28232182
  6. Our data collectively indicate that miR-613 functions as a tumor suppressor in retinoblastoma through downregulating E2F5, supporting the targeting of the novel miR-613/E2F5 axis as a potentially effective therapeutic approach for retinoblastoma. PMID: 28351331
  7. MiR-34a was down-regulated in colorectal cancer cells and inversely correlated with FMNL2 and E2F5 expressions. Our study suggests that miR-34a is an important tumor suppressor of CRC progression by targeting FMNL2 and E2F5. PMID: 26103003
  8. miR-98 regulates muscle differentiation by altering the expression of the transcription factor E2F5 and, in turn, of multiple E2F5 targets. PMID: 25422988
  9. In hepatocellular carcinoma cells hepatitis B virus down-regulates E2F5 expression by up-regulating expression of miR-181a. PMID: 24529171
  10. expression of E2F5 in esphageal squamous cell cancer (ESCC) may be correlated with a worse prognosis of patients with ESCC PMID: 24324077
  11. E2F5 may be a novel potential candidate marker for malignant prostate cancer PMID: 23377984
  12. Data indicate that E2F5 is commonly overexpressed in primary hepatocellular carcinomas and show that E2F5 knockdown significantly repressed the growth of HCC cells. PMID: 21274376
  13. cell-cycle regulatory protein E2F5 might play a significant role in epithelial ovarian cancer pathogenesis PMID: 20181230
  14. Diverting the function of E2F5 from a cell-cycle repressor into an activator might contribute to the higher oncogenic potential of HPV18 when compared with other high-risk HPV types. PMID: 20639900
  15. the subcellular distribution of E2F-5 is tightly regulated in intact cells, through multiple functional domains that direct nucleocytoplasmic shuttling of this protein. PMID: 12089160
  16. EBV LPM1 blocks p16INK4 pathway by promoting nuclear export of E2F-5. PMID: 12860972
  17. E2F5 protein is down-regulated late in embryogenesis PMID: 16172982
  18. Overexpression of E2F5/p130 in post-mitotic lens fiber cells does not affect normal differentiation, but can inhibit inappropriate cell cycle reentry induced by activator E2Fs. P130 is key player in inhibitory process. PMID: 18385796
  19. Overexpression of E2F-5 correlates with a pathological basal phenotype and a worse clinical outcome. PMID: 19259095

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Subcellular Location Nucleus.
Protein Families E2F/DP family
Database Links

HGNC: 3119

OMIM: 600967

KEGG: hsa:1875

STRING: 9606.ENSP00000398124

UniGene: Hs.445758

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