E2F5 Antibody

Code CSB-PA624021LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, Rat liver tissue, Mouse liver tissue
    All lanes: E2F5 antibody at 5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 38, 21 kDa
    Observed band size: 38 kDa

  • IHC image of CSB-PA624021LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) E2F5 Polyclonal antibody
Uniprot No. Q15329
Target Names E2F5
Alternative Names E2F 5 antibody; E2F transcription factor 5 antibody; E2F transcription factor 5 p130 binding antibody; E2F-5 antibody; E2f5 antibody; E2F5_HUMAN antibody; p130 binding antibody; Transcription factor E2F5 antibody
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human Transcription factor E2F5 protein (193-298AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation
Gene References into Functions
  1. High E2F5 expression is associated with Glioma. PMID: 29362021
  2. knockdown of E2F5 and PFTK1 mimicked the tumor-suppressive effects of miR-1-3p overexpression on PCa progression. Conversely, concomitant knockdown of miR-1-3p and E2F5 and PFTK1 substantially reversed the inhibitory effects of either E2F5 or PFTK1 silencing alone. PMID: 30185212
  3. miR-1179 overexpression could also inhibit tumor growth in vivo by suppressing the expression of E2F5. PMID: 29859832
  4. FOXN3 functions as a tumor suppressor in hepatocellular carcinoma by downregulating the expression of E2F5. PMID: 27259277
  5. Taken together, these findings indicated that SNHG16 induces breast cancer cell migration by competitively binding miR-98 with E2F5. PMID: 28232182
  6. Our data collectively indicate that miR-613 functions as a tumor suppressor in retinoblastoma through downregulating E2F5, supporting the targeting of the novel miR-613/E2F5 axis as a potentially effective therapeutic approach for retinoblastoma. PMID: 28351331
  7. MiR-34a was down-regulated in colorectal cancer cells and inversely correlated with FMNL2 and E2F5 expressions. Our study suggests that miR-34a is an important tumor suppressor of CRC progression by targeting FMNL2 and E2F5. PMID: 26103003
  8. miR-98 regulates muscle differentiation by altering the expression of the transcription factor E2F5 and, in turn, of multiple E2F5 targets. PMID: 25422988
  9. In hepatocellular carcinoma cells hepatitis B virus down-regulates E2F5 expression by up-regulating expression of miR-181a. PMID: 24529171
  10. expression of E2F5 in esphageal squamous cell cancer (ESCC) may be correlated with a worse prognosis of patients with ESCC PMID: 24324077
  11. E2F5 may be a novel potential candidate marker for malignant prostate cancer PMID: 23377984
  12. Data indicate that E2F5 is commonly overexpressed in primary hepatocellular carcinomas and show that E2F5 knockdown significantly repressed the growth of HCC cells. PMID: 21274376
  13. cell-cycle regulatory protein E2F5 might play a significant role in epithelial ovarian cancer pathogenesis PMID: 20181230
  14. Diverting the function of E2F5 from a cell-cycle repressor into an activator might contribute to the higher oncogenic potential of HPV18 when compared with other high-risk HPV types. PMID: 20639900
  15. the subcellular distribution of E2F-5 is tightly regulated in intact cells, through multiple functional domains that direct nucleocytoplasmic shuttling of this protein. PMID: 12089160
  16. EBV LPM1 blocks p16INK4 pathway by promoting nuclear export of E2F-5. PMID: 12860972
  17. E2F5 protein is down-regulated late in embryogenesis PMID: 16172982
  18. Overexpression of E2F5/p130 in post-mitotic lens fiber cells does not affect normal differentiation, but can inhibit inappropriate cell cycle reentry induced by activator E2Fs. P130 is key player in inhibitory process. PMID: 18385796
  19. Overexpression of E2F-5 correlates with a pathological basal phenotype and a worse clinical outcome. PMID: 19259095

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Subcellular Location Nucleus
Protein Families E2F/DP family
Database Links

HGNC: 3119

OMIM: 600967

KEGG: hsa:1875

STRING: 9606.ENSP00000398124

UniGene: Hs.445758

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