Code | CSB-YP023924HU |
Size | $306 |
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The recombinant active human Transmembrane protease serine 2 (TMPRSS2) is produced by the expression of a target DNA sequence with 6xHis, N-terminal tag(s), in the yeast expression system. The target DNA sequence encodes the 106-492aa region of the human TMPRSS2. The purity of this partial-length protein is greater than 85% determined by SDS-PAGE. The gel showed a molecular weight band of about 45 kDa under reducing conditions. And its enzymatic activity was verified by its ability to cleave fluorogenic peptide substrate (Boc-Gln-Ala-Arg-AMC) (Km is 21.93μM).
The protease TMPRSS2 plays an important role in the infection mechanism of human coronaviruses, such as SARS-CoV and SARS-CoV-2. Cell entry of human coronaviruses depends on the binding of the viral spike (S) glycoprotein to cellular ACE2 receptor and S protein priming by host cell protease TMPRSS2. Moreover, TMPRSS2 protein is commonly used to study cancer. Previous studies have shown that the TMPRSS2 gene was up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue.
Applications : Enzymatic activity in vitro
Review: CSB-YP023924HU- TMPRSS2 - Lot DA04630a6g0 works so we will ordering the remaining 950ug of the same lot.
By Anonymous
Applications : Enzymatic activity in vitro
Review: We tested the protein and is having positive preliminary results.
By Anonymous
Applications : Enzymatic activity in vitro
Review: The enzyme demonstrates good relative activity in our preliminary testing, and should allow us to complete our first round of proposed screening. Once completed, that screening data will be posted on our Open Data Portal (https://opendata.ncats.nih.gov/covid19/), which will include assay details and list you as the source of the enzyme, which I trust will be welcomed by your sales team. To support further screening, we would like to order more, and kindly ask for a new quote for 5mgs, along with an estimated timeline, if possible.
By Anonymous
Applications : Enzymatic activity in vitro
Review: Can you let me know the availability of catalog: CSB-YP023924HU? We would be interested in ordering either 1 or 2 x 100ug or 1mg. Can you let me know if lot DA04585a6g0 is available to fulfill those orders? We previously received this lot in early August which shows good activity and would like to receive this lot again if it is available.
By Anonymous
Applications : Surface plasmon resonance (SPR)
Review: TMPRSS2 using pERTKR-AMC or Boc-QAR-AMC respectively. 100 µM of compound, or vehicle control (DMSO), was incubated with the proteases for 30 minutes prior to addition of substrate and detection of fluorescent activity.
By Anonymous
Applications : antiviral potential of atovaquone
Review: Atovaquone restricts wtVSV and VSV-SARS spike infectivity in VeroE6 and VeroE6 hTMPRSS2-hACE2 cells. VeroE6 cells or VeroE6 cells stably transduced with hACE2 and TMPRSS2 were seeded at 2.5 × 104 cells/cm2 in 96-well plates and treated with various concentrations of atovaquone.
By Anonymous
Applications : Western blot
Review: TMPRSS2-mediated S-protein cleavage in the presence or absence of individual serpins and the known protease inhibitor nafamostat mesylate. Data from three independent experiments were quantified, and a representative blot is shown (E). The intensity of bands in panel E corresponding to cleaved S-protein was quantified using ImageJ (Fuji) and normalized to S-protein and TMPRSS2 control. Mean values and SEM are shown; statistical significance was calculated by unpaired t test (*, P , 0.05; **, P , 0.01) (F).
By Anonymous
Applications : luciferase activity assay
Review: Infectivity of PsVs with different point mutations in CS-1 and CS-2 was assessed in 293 T/ACE2 cells with CTSL (293 T/CTSL), TMPRSS2 (293 T/TMPRSS2), and FURIN (293 T/FURIN) genes overexpression. PsV infectivity was measured by a luciferase assay and is shown as the raw luciferase activity (n = 3–4). Infectivity of PsVs at the indicated fold dilutions in in LLC-MK2, Vero, Huh7, 293T/ACE2 cells and in 293T/ACE2 cells with CTSL (293T/CTSL), TMPRSS2 (293T/TMPRSS2) and FURIN (293T/FURIN) genes overexpression. (n = 4).
By Anonymous
Applications : Drug related studies
Review: The compounds against 30 nM of human TMPRSS2 using fluorogenic substrate 100 µM Boc-Gln-Ala-Arg-AMC; however, there was no inhibitory activity of any compound up to 100 μM against serine protease TMPRSS2.
By Anonymous
We will require this recombinant TMPRSS2 protein to set up the assays and to screen the chemical library. So, please suggest which one I should choose, Yeast derived or E coli derived?
What QC test will you perform?
What is the general protectant? What kind of protectant do you usually add?
When making TMPRSS2, do you use any protein denaturing purification steps?
Is TMPRSS2 soluble in water (buffer) or is it necessary to resuspend products in a solvent such as DMSO?
Was it difficult to manufacture TMPRSS2?
Do you know whether this product has been tested for activity?
I want to order 500 μg of CSB-YP023924HU. Would you let me know what form of the protein is provided (liquid or powder)?
Can you remove the endotoxin?
What is the general preservative? Which kind of preservative do you usually add?