Recombinant Human Transmembrane protease serine 2 (TMPRSS2), Biotinylated, is produced in E. coli and contains amino acids 106-492 of the human protein. This partial protein carries an N-terminal MBP tag and a C-terminal 6xHis-Avi tag, which allows for various experimental applications. The product shows purity greater than 85% as determined by SDS-PAGE, meeting quality standards for research use.
TMPRSS2 is a serine protease that participates in various biological processes and appears to play a key role in proteolytic processing. It's involved in cleaving specific proteins and takes part in important cellular pathways, including those related to viral entry mechanisms. Because of its involvement in these critical pathways, TMPRSS2 has become a significant focus of research in several biochemical and biomedical studies.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protease Inhibitor Screening and Characterization
This recombinant TMPRSS2 protein may serve as a target for screening potential serine protease inhibitors in vitro. The biotinylated protein allows immobilization on streptavidin-coated surfaces for high-throughput screening assays. Researchers can assess the binding affinity and specificity of small molecule compounds or peptide-based inhibitors. The dual tagging system (MBP and His) offers additional purification and detection options for biochemical assays.
2. Protein-Protein Interaction Studies
The biotinylated TMPRSS2 can be used in pull-down assays to identify and characterize protein binding partners or substrates. The biotin tag allows for efficient capture using streptavidin beads, while the His tag provides alternative purification strategies. This approach is valuable for mapping TMPRSS2 interaction networks and understanding its role in cellular proteolytic cascades. The expressed region (106-492aa) represents a substantial portion of the protein that likely retains important binding domains.
3. Antibody Development and Validation
This recombinant protein serves as an immunogen and target for developing TMPRSS2-specific antibodies. The high purity (>85%) and substantial protein length make it suitable for immunization protocols in antibody production. The biotinylated protein can be used in ELISA-based assays to screen and validate antibody specificity and binding affinity. Multiple tags provide versatile detection and capture options for various immunoassay formats.
4. Structural and Biochemical Characterization Studies
The recombinant TMPRSS2 protein can be used for biophysical studies to understand its structural properties and stability. The MBP tag may enhance protein solubility and folding, making it suitable for techniques such as dynamic light scattering, circular dichroism spectroscopy, or analytical ultracentrifugation. Researchers can investigate the protein's conformational changes under different buffer conditions or in response to potential binding partners. The biotinylation enables surface-based analytical techniques for real-time binding kinetics studies.
