Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TPM3

Uniprot No.

P06753

Research Area

Signal Transduction

Alternative Names

Alpha tropomyosin 3; Alpha tropomyosin slow skeletal; CFTD; Cytoskeletal tropomyosin TM30; FLJ41118; gamma TM; Gamma tropomyosin; Gamma-tropomyosin; Heat stable cytoskeletal protein 30 kDa; hscp30; hTM30nm; hTM5; hTMnm; MGC102590; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; OTTHUMP00000034019; OTTHUMP00000034171; OTTHUMP00000034172; TM 5; TM-5; TM3; TM30; TM30nm; TM5; Tm5NM; Tpm 5; TPM3; TPM3/NTRK1 FUSION GENE, INCLUDED; TPM3_HUMAN; Tpm5; TPMsk3; TRK; TRK ONCOGENE, INCLUDED; Trop 5; Tropomyosin 3; Tropomyosin 3 gamma; Tropomyosin 5; Tropomyosin alpha 3 chain; Tropomyosin alpha-3 chain; Tropomyosin gamma; Tropomyosin-3; Tropomyosin-5

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-285aa

Target Protein Sequence

MEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISEELDHALNDMTSI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

59.8kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human TPM3 protein is produced by constructing the recombinant plasmid encoding the Human TPM3 protein (2-285aa), transforming recombinant plasmid into e.coli cells, screening the positive e.coli cells and culturing them, and inducing the protein expression. The protein is equipped with a N-terminal GST tag. Following expression, the recombinant TPM3 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human TPM3 protein. Its purity is greater than 85%.

Target Background

Function

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Gene References into Functions

Analysis of the residual, resected tumor identified a chromoplectic TPM3-ALK rearrangement that involved many other known oncogenes and was confirmed by rtPCR. PMID: 27742657
expression levels of tropomyosin 3 (TPM3) were higher in stage III ESCC tissue compared with stage I (P<0.05). The findings of the present study identified twelve proteins, which are closely associated with ESCC invasion and metastasis, apoptosis and cell signal transduction. PMID: 28138712
Dominant mutations in TPM3, encoding alpha-tropomyosinslow, cause a congenital myopathy characterized by generalized muscle weakness. Here, we used a multidisciplinary approach to investigate the mechanism of muscle dysfunction in 12 TPM3-myopathy patients. PMID: 26307083
This work expands the phenotypic spectrum of TPM3-related disease and provides insights into the pathophysiological mechanisms of the actin-tropomyosin complex PMID: 26418456
Western blot showed phosphorylation of ALK, ERK1/2, and STAT3 in cells transfected with TPM3-ALK. Coiled-coil structure of TPM3 contributes to the transforming ability of the TPM3-ALK fusion protein, and longer TPM3 region leads to higher dimer formation. PMID: 25596129
DATA show that tropomyosin 3 protein (TPM3) plays a critical role in the progression of gliomas. PMID: 24913705
study reports on a three-generation family with cap myopathy caused by a novel heterozygous mutation in TPM3 PMID: 24239060
TPM3-R167H mutations decreased cooperative thin filament activation in combination with reductions in the myosin cross-bridge number and force production. PMID: 22798622
TPM3 is an interacting partner of granulin-epithelin precursor and may play an important role in hepatocarcinogenesis. PMID: 22792281
investigation of biomarkers for early diagnosis of endometriosis: Data suggest that TPM3, stomatin-like protein 2, and tropomodulin 3 are autoantigens present in blood of women with endometriosis; immunodominant epitopes were identified. PMID: 22158085
study reports clinico-pathological and electrophysiological features of 2 unrelated cases with heterozygous TPM3 mutation; cases highlight neuromuscular transmission defect in congenital myopathy with fibre type disproportion secondary to TPM3 mutations PMID: 20951040
High TPM3-PDGFRB fusion protein expression is associated with chronic eosinophilic leukemia. PMID: 21072821
variation in the tropomyosin isoform composition of microfilaments provides a mechanism to generate functionally distinct filament populations PMID: 21036167
TPM3 mutations are involved in fiber size disproportion in congenital myotonic dystrophy PMID: 20179953
the clinical, myopathological and muscle MRI findings in a German family with autosomal dominant nemaline myopathy due to a novel pathogenic TPM3 mutation PMID: 20012312
Overexpression of TPM3 activates Snail mediated EMT, which will repress E-cadherin expression and that it confers migration or invasion potentials to HCC cells during hepatocarcinogenesis. PMID: 20356415
Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia. PMID: 20223922
Mutations in TPM3 were identified in 6 out of 13 patients with Congenital fiber type disproportion, as well as in one case of nemaline myopathy. PMID: 19953533
cloned and sequenced a novel nonmuscle tropomyosin (hTM) isoform, TC22, which is strongly associated with colonic neoplasia and carcinoma PMID: 12105844
A mutation converting the stop codon to a serine and a second splicing mutation predicted to prevent inclusion of skeletal muscle exon IX were found associated with nemaline myopathy PMID: 12196661
De novo missense mutation in a constitutively expressed exon of the slow alpha-tropomyosin gene TPM3 associated with an atypical, sporadic case of nemaline myopathy. PMID: 12467750
tropomyosin isoforms regulate neuronal size and shape PMID: 15888546
second pedigree with autosomal dominant nemaline myopathy caused by TPM3 mutation(Arg167His). PMID: 17376686
Mutation of TPM3 is the most common cause of congenital fiber type disproportion reported to date. PMID: 18300303
The mutation reported here is the first deletion to be identified in TPM3, and it is likely to be a founder mutation in the Turkish population. PMID: 18382475
TTC9A acts as a chaperone protein to facilitate the function of tropomyosins (including Tm5NM-1) in stabilizing microfilament and it may play a role in cancer cell invasion and metastasis PMID: 18699990
We report a TPM3 mutation in one of the original cases of cap disease. PMID: 19487656

Hide All

Involvement in disease

Nemaline myopathy 1 (NEM1); Myopathy, congenital, with fiber-type disproportion (CFTD); Cap myopathy 1 (CAPM1)

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

Tropomyosin family

Database Links

HGNC: 12012

OMIM: 164970

KEGG: hsa:7170

STRING: 9606.ENSP00000357516

UniGene: Hs.535581

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Code	CSB-EP024106HU
Abbreviation	Recombinant Human TPM3 protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Tropomyosin alpha-3 chain (TPM3)

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