Recombinant Human Troponin T, fast skeletal muscle (TNNT3)

Code CSB-YP024017HU
MSDS
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Source Yeast
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Code CSB-EP024017HU
MSDS
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Source E.coli
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Code CSB-EP024017HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP024017HU
MSDS
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Source Baculovirus
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Code CSB-MP024017HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TNNT3
Uniprot No.
Alternative Names
AMCD2B; Beta TnTF; Beta-TnTF; DA2B; DKFZp779M2348; fast skeletal muscle; Fast skeletal muscle troponin T; FSSV; fTnT; TNNT 3; Tnnt3; TNNT3_HUMAN; TnTf; Troponin T; Troponin T fast skeletal muscle; Troponin T type 3 (skeletal fast); Troponin T type 3; Troponin T3 skeletal fast
Species
Homo sapiens (Human)
Expression Region
2-269
Target Protein Sequence
SDEEVEQVE EQYEEEEEAQ EEAAEVHEEV HEPEEVQEDT AEEDAEEEKP RPKLTAPKIP EGEKVDFDDI QKKRQNKDLM ELQALIDSHF EARKKEEEEL VALKERIEKR RAERAEQQRI RAEKERERQN RLAEEKARRE EEDAKRRAED DLKKKKALSS MGANYSSYLA KADQKRGKKQ TAREMKKKIL AERRKPLNID HLGEDKLRDK AKELWETLHQ LEIDKFEFGE KLKRQKYDIT TLRSRIDQAQ KHSKKAGTPA KGKVGGRWK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. The presence of the p.(Arg63His) missense mutation at position 63 of TNNT3 was confirmed through direct cycle sequencing of genomic DNA in six affected South African family members for whom DNA had been archived. PMID: 26915936
  2. Data show that a missense mutation at nucleotide position 187 in exon 9 of the TNNT3 gene was identified in all affected individuals in the family. PMID: 21402185
  3. TNNT3 and LDB3 showed abnormal splicing and appeared more pronounced in myotonic dystrophies type 2 relative to myotonic dystrophies type 1. PMID: 20066428
  4. These results indicate that the f peptide confers an inhibitory effect on the biological function of fast skeletal troponin T and this can be correlated with changes in the Ca2+ regulation associated with development in fast skeletal muscle. PMID: 16081096
  5. Skeletal muscle contractile gene (TNNT3, MYH3, TPM2) mutations not found in vertical talus or clubfoot. PMID: 19142688
  6. Myotonic dystrophy type 2 muscles exhibited a higher degree of alternative splicing dysregulation for fast TnT transcripts when compared to myotonic dystrophy type 1 muscles. PMID: 19326042

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Involvement in disease
Arthrogryposis, distal, 2B (DA2B)
Protein Families
Troponin T family
Tissue Specificity
In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
Database Links

HGNC: 11950

OMIM: 600692

KEGG: hsa:7140

STRING: 9606.ENSP00000278317

UniGene: Hs.73454

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