Recombinant Human Type-1 angiotensin II receptor-associated protein (AGTRAP), partial

Code CSB-YP744194HU1
MSDS
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Source Yeast
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Code CSB-EP744194HU1
MSDS
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Source E.coli
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Code CSB-EP744194HU1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744194HU1
MSDS
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Source Baculovirus
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Code CSB-MP744194HU1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGTRAP
Uniprot No.
Alternative Names
AGTRAP; ATRAP; Type-1 angiotensin II receptor-associated protein; AT1 receptor-associated protein
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity.
Gene References into Functions
  1. A role for AT1 receptor-associated proteins in blood pressure regulation PMID: 25562590
  2. ATRAP, a directly interacting and functionally inhibiting molecule of AT1R, plays a protective role against the development of systemic insulin resistance via regulatory effects on adipose tissue function. PMID: 23902639
  3. The phosphatidylinositol transfer protein RdgBbeta binds 14-3-3 via its unstructured C-terminus, whereas its lipid-binding domain interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein). PMID: 21728994
  4. the GG genotype of the AGTRAP rs11121816 T/G single nucleotide polymorphism was associated with increased mortality in two cohorts of patients who had septic shock. PMID: 21423001
  5. CSF angiotensin II, ACE, and ACE2 levels are decreased in neuromyelitis optica/NMO spectrum disorder patients with anti-AQP4 antibody, reflecting severe destruction of perivascular astrocytes PMID: 20541774
  6. Endogenous increases in angiotensin II during dehydration contribute to dehydration-associated sympathoexcitation. PMID: 19805740
  7. Small interfering RNA significantly attenuates angiotensin II type 1 receptor-stimulated inositol phosphate formation. PMID: 20018811
  8. cloned a new human gene cDNA that codes for a homolog of the murine Agtrap protein PMID: 11733189
  9. isolation of a novel protein, ARAP1, which promotes recycling of angiotensin(1A) to the plasma membrane in HEK-293 cells PMID: 14559250
  10. mRNA is detected in all human tissues; cellular hypertrophy in cultured rat vascular smooth muscle and renal proximal tubular cells. PMID: 14985364
  11. CAML is an important signal transducer for the actions of Ang II in regulating the calcineurin-NFAT pathway and the interaction of CAML with ATRAP may mediate the Ang II actions in vascular physiology PMID: 15668245

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Present in perinuclear vesicular membranes, Endoplasmic reticulum, Golgi and endocytic vesicles.
Tissue Specificity
Ubiquitous but more abundant in kidney, heart, pancreas and thyroid.
Database Links

HGNC: 13539

OMIM: 608729

KEGG: hsa:57085

STRING: 9606.ENSP00000319713

UniGene: Hs.464438

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