Recombinant Human Tyrosine-protein kinase ITK/TSK(ITK)

Code CSB-YP600145HU
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Source Yeast
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Code CSB-EP600145HU
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Source E.coli
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Code CSB-EP600145HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP600145HU
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Source Baculovirus
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Code CSB-MP600145HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ITK
Uniprot No. Q08881
Alternative Names EMT; Homolog of mouse T cell itk/tsk; IL 2 inducible T cell kinase; IL2 inducible T cell kinase; Interleukin 2 inducible T cell kinase; Itk; ITK_HUMAN; Kinase EMT; LPFS1; LYK; MGC126257; MGC126258; PSCTK 2; PSCTK2; T cell specific kinase; T-cell-specific kinase; TSK; Tyrosine protein kinase; Tyrosine protein kinase ITK/TSK; Tyrosine protein kinase Lyk; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk
Species Homo sapiens (Human)
Expression Region 1-620
Target Protein Sequence MNNFILLEEQ LIKKSQQKRR TSPSNFKVRF FVLTKASLAY FEDRHGKKRT LKGSIELSRI KCVEIVKSDI SIPCHYKYPF QVVHDNYLLY VFAPDRESRQ RWVLALKEET RNNNSLVPKY HPNFWMDGKW RCCSQLEKLA TGCAQYDPTK NASKKPLPPT PEDNRRPLWE PEETVVIALY DYQTNDPQEL ALRRNEEYCL LDSSEIHWWR VQDRNGHEGY VPSSYLVEKS PNNLETYEWY NKSISRDKAE KLLLDTGKEG AFMVRDSRTA GTYTVSVFTK AVVSENNPCI KHYHIKETND NPKRYYVAEK YVFDSIPLLI NYHQHNGGGL VTRLRYPVCF GRQKAPVTAG LRYGKWVIDP SELTFVQEIG SGQFGLVHLG YWLNKDKVAI KTIREGAMSE EDFIEEAEVM MKLSHPKLVQ LYGVCLEQAP ICLVFEFMEH GCLSDYLRTQ RGLFAAETLL GMCLDVCEGM AYLEEACVIH RDLAARNCLV GENQVIKVSD FGMTRFVLDD QYTSSTGTKF PVKWASPEVF SFSRYSSKSD VWSFGVLMWE VFSEGKIPYE NRSNSEVVED ISTGFRLYKP RLASTHVYQI MNHCWKERPE DRPAFSRLLR QLAEIAESGL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3.
Gene References into Functions
  1. These observations have significant therapeutic implications, as pharmacologic inhibition of ITK prevented the activation of this signaling axis and overcame chemotherapy resistance. PMID: 27780854
  2. Our results provide new insight into the effect of ITK and suboptimal T-cell receptor signaling on CD8(+) T cell function, and how these may contribute to phenotypes associated with ITK deficiency PMID: 28213500
  3. The data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm. PMID: 27729219
  4. We conclude that ITK, formerly considered an immune cell-specific protein, is aberrantly expressed in melanoma and promotes tumor development and progression PMID: 25934889
  5. The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192. PMID: 25492967
  6. Found that 38% and 14% of the Angioimmunoblastic T-cell lymphoma cases exhibited gains of ITK and SYK genes, respectively. PMID: 25337257
  7. These data indicate that PRN694 is a highly selective and potent covalent inhibitor of ITK and RLK, and its extended target residence time enables durable attenuation of effector cells in vitro and in vivo. PMID: 25593320
  8. approach identified 18 kinase and kinase-related genes whose overexpression can substitute for EGFR in EGFR-dependent PC9 cells, and these genes include seven of nine Src family kinase genes, FGFR1, FGFR2, ITK, NTRK1, NTRK2, MOS, MST1R, and RAF1. PMID: 25512530
  9. ITK deficiency is a genetic cause of idiopathic CD4+ T-cell lymphopenia. PMID: 25061172
  10. Data indicate reduced T-cell activation by altered IL-2 inducible T-cell kinase (ITK) expression in vitro. PMID: 24462896
  11. T-cell-specific human ITK-Syk oncogene in mice leads to early polyclonal T cell lymphoproliferation with B cell expansion. It induces terminal T cell differentiation via Blimp-1, eliminating oncogene-expressing cells early in development. PMID: 24376268
  12. Case Report/Letter: ITK/SYK translocation in angioimmunoblastic T-cell lymphoma. PMID: 24076779
  13. a new pathway regulated by Itk in cells, and suggest cross talk between Itk and G-protein signaling downstream of the TcR. PMID: 23454662
  14. Data indicate that the intracellular signaling of ITK-SYK requires both SLP-76 and the adapter function of SYK/ZAP-70 kinases. PMID: 23293025
  15. ITK and Gag colocalized at the plasma membrane and were concentrated at sites of F-actin accumulation and membrane lipid rafts in HIV-1 infected T cells PMID: 23260110
  16. DEF6, a novel substrate for the Tec kinase ITK, contains a glutamine-rich aggregation-prone region and forms cytoplasmic granules that co-localize with P-bodies. PMID: 22829599
  17. This review focuses on Itk and its role in regulating T-cell signaling and function, especially the activation and development of alpha-beta T cells. PMID: 22449075
  18. ITK mutations are distributed over the entire protein and include missense, nonsense and indel mutations, reminiscent of the situation in its sister kinase in B cells, Bruton's tyrosine kinase PMID: 22289921
  19. These results indicate that Itk is required for efficient replication of influenza virus in infected T-cells. PMID: 22302878
  20. No detrimental mutations were identified in ITK in Chinese children with Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis. PMID: 21674762
  21. These data suggest a sequential mechanism whereby ZAP-70-dependent priming of SLP-76 at three N-terminal sites triggers reciprocal regulatory interactions between Itk and SLP-76, which are ultimately required to couple active Itk to PLC-gamma1. PMID: 21725281
  22. People with the -rare allele 196C>T may be more susceptible to asthma via transcriptional regulation of the ITK gene. PMID: 21323647
  23. Knocking down Itk expression inhibits Jurkat cell proliferation and cytokines production. PMID: 21280324
  24. TSAd, through its interaction with both Itk and Lck, primes Itk for Lck mediated phosphorylation and thereby regulates CXCL12 induced T cell migration and actin cytoskeleton rearrangements PMID: 20305788
  25. T-cell receptor (TCR)-induced association between ITK and SLP-76, recruitment and transphosphorylation of ITK, actin polarization at the T-cell contact site, and expression of Th2 cytokines PMID: 20457812
  26. Expression of patient-derived ITK in mice induces highly malignant peripheral T cell lymphomas. PMID: 20439541
  27. Data show that tacrolimus modified the expression levels of Foxp3-regulated T cell receptor signal related-genes, PTPN22 and Itk, in Treg cells. PMID: 19714314
  28. Itk catalytic activity is inhibited by the peptidyl prolyl isomerase activity of cyclophilin A (CypA). PMID: 11830645
  29. In this review, the pathways are discussed by which Itk might impact the differentiation of T helper (Th) cells. PMID: 16931156
  30. Itk forms dimers in the membrane and that receptors that recruit Itk do so to specific membrane regions PMID: 17060314
  31. Vav phosphorylation correlates with calcium flux and Itk phosphorylation during mitogenic CD28 signalaing in Jurkat cells PMID: 17237383
  32. Active signals from transgenic Tec kinase (ITK) regulate the development of memory-like CD8+ T cells with innate function. PMID: 17724684
  33. Itk autophosphorylation on Y180 within the SH3 domain occurs exclusively via an intramolecular, in cis mechanism PMID: 17897671
  34. In transgenic mice specifically lacking Itk kinase activity, active kinase signaling is required for control of T helper (Th)2 cell responses and development of allergic asthma. PMID: 18322190
  35. These data suggest that inhibition of ITK blocks HIV infection by affecting multiple steps of HIV replication. PMID: 18443296
  36. Analysis of SNPs in TCR pathway genes revealed that a haplotype that covers a major part of the coding sequence of ITK is a risk factor for seasonal allergic rhinitis. PMID: 19222422
  37. ITK deficiency as a result of a R335W missense mutation, causes a novel immunodeficiency syndrome that leads to a fatal inadequate immune response to Epstein-Barr virus. PMID: 19425169
  38. The authors identify the residues on the surface of the Itk SH2 domain responsible for substrate docking and show that this SH2 surface mediates autophosphorylation in the full-length Itk molecule. PMID: 19523959
  39. Findings identify ITK-SYK as an active, transforming FTK and suggest ITK-SYK as a rational therapeutic target for t(5;9)(q33;q22)-positive lymphomas. PMID: 19535334
  40. these results provide an initial step in understanding the biological role of Itk-TFII-I signaling in T-cell function. PMID: 19701889
  41. The Tec family kinase Itk exists as a folded monomer in vivo. PMID: 19717557
  42. Studies of Itk deletion mutants indicate that the amino acids in the N-terminal 152 residues and proline-rich domains enhance catalysis by affecting turnover rate rather than by substrate binding. PMID: 11437596

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Involvement in disease Lymphoproliferative syndrome 1 (LPFS1)
Subcellular Location Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
Protein Families Protein kinase superfamily, Tyr protein kinase family, TEC subfamily
Tissue Specificity T-cell lines and natural killer cell lines.
Database Links

HGNC: 6171

OMIM: 186973

KEGG: hsa:3702

STRING: 9606.ENSP00000398655

UniGene: Hs.558348

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