Product Details

Uniprot No.

Target Names

Alternative Names

Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2) (Interleukin-2-inducible T-cell kinase) (IL-2-inducible T-cell kinase) (Kinase EMT) (T-cell-specific kinase) (Tyrosine-protein kinase Lyk), ITK, EMT LYK

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ITK

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ITK, or interleukin-2-inducible T-cell kinase, serves as a critical non-receptor tyrosine kinase that orchestrates T-cell receptor signaling pathways essential for adaptive immune responses. This kinase plays a pivotal role in T-cell development, activation, and differentiation, making it a valuable target for researchers investigating immune cell biology, autoimmune disorders, and T-cell mediated diseases.

This recombinant monoclonal antibody, clone 8F10, offers the reproducibility and consistency that demanding immunological research requires. Generated against a synthetic peptide derived from human ITK and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The result is reliable, reproducible data across extended studies and between laboratories.

Validation in immunohistochemistry demonstrates clear detection in paraffin-embedded human lymph node tissue, a particularly relevant sample type given ITK's predominant expression in T-cells and the lymphoid compartment. Using a Leica Bond system with citrate buffer antigen retrieval and detection via HRP-conjugated secondary antibody, the antibody performs effectively at dilutions between 1:50 and 1:200, giving researchers flexibility to optimize staining intensity for their specific tissue preparations. The antibody is also validated for ELISA applications, expanding its utility across different experimental workflows.

Supplied in a glycerol-containing buffer optimized for long-term stability, this affinity-purified antibody arrives ready for immediate use in signal transduction research, T-cell biology studies, and investigations into immune-related pathologies where ITK function is implicated.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3.

Gene References into Functions

These observations have significant therapeutic implications, as pharmacologic inhibition of ITK prevented the activation of this signaling axis and overcame chemotherapy resistance. PMID: 27780854
Our results provide new insight into the effect of ITK and suboptimal T-cell receptor signaling on CD8(+) T cell function, and how these may contribute to phenotypes associated with ITK deficiency PMID: 28213500
The data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm. PMID: 27729219
We conclude that ITK, formerly considered an immune cell-specific protein, is aberrantly expressed in melanoma and promotes tumor development and progression PMID: 25934889
The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192. PMID: 25492967
Found that 38% and 14% of the Angioimmunoblastic T-cell lymphoma cases exhibited gains of ITK and SYK genes, respectively. PMID: 25337257
These data indicate that PRN694 is a highly selective and potent covalent inhibitor of ITK and RLK, and its extended target residence time enables durable attenuation of effector cells in vitro and in vivo. PMID: 25593320
approach identified 18 kinase and kinase-related genes whose overexpression can substitute for EGFR in EGFR-dependent PC9 cells, and these genes include seven of nine Src family kinase genes, FGFR1, FGFR2, ITK, NTRK1, NTRK2, MOS, MST1R, and RAF1. PMID: 25512530
ITK deficiency is a genetic cause of idiopathic CD4+ T-cell lymphopenia. PMID: 25061172
Data indicate reduced T-cell activation by altered IL-2 inducible T-cell kinase (ITK) expression in vitro. PMID: 24462896
T-cell-specific human ITK-Syk oncogene in mice leads to early polyclonal T cell lymphoproliferation with B cell expansion. It induces terminal T cell differentiation via Blimp-1, eliminating oncogene-expressing cells early in development. PMID: 24376268
Case Report/Letter: ITK/SYK translocation in angioimmunoblastic T-cell lymphoma. PMID: 24076779
a new pathway regulated by Itk in cells, and suggest cross talk between Itk and G-protein signaling downstream of the TcR. PMID: 23454662
Data indicate that the intracellular signaling of ITK-SYK requires both SLP-76 and the adapter function of SYK/ZAP-70 kinases. PMID: 23293025
ITK and Gag colocalized at the plasma membrane and were concentrated at sites of F-actin accumulation and membrane lipid rafts in HIV-1 infected T cells PMID: 23260110
DEF6, a novel substrate for the Tec kinase ITK, contains a glutamine-rich aggregation-prone region and forms cytoplasmic granules that co-localize with P-bodies. PMID: 22829599
This review focuses on Itk and its role in regulating T-cell signaling and function, especially the activation and development of alpha-beta T cells. PMID: 22449075
ITK mutations are distributed over the entire protein and include missense, nonsense and indel mutations, reminiscent of the situation in its sister kinase in B cells, Bruton's tyrosine kinase PMID: 22289921
These results indicate that Itk is required for efficient replication of influenza virus in infected T-cells. PMID: 22302878
No detrimental mutations were identified in ITK in Chinese children with Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis. PMID: 21674762
These data suggest a sequential mechanism whereby ZAP-70-dependent priming of SLP-76 at three N-terminal sites triggers reciprocal regulatory interactions between Itk and SLP-76, which are ultimately required to couple active Itk to PLC-gamma1. PMID: 21725281
People with the -rare allele 196C>T may be more susceptible to asthma via transcriptional regulation of the ITK gene. PMID: 21323647
Knocking down Itk expression inhibits Jurkat cell proliferation and cytokines production. PMID: 21280324
TSAd, through its interaction with both Itk and Lck, primes Itk for Lck mediated phosphorylation and thereby regulates CXCL12 induced T cell migration and actin cytoskeleton rearrangements PMID: 20305788
T-cell receptor (TCR)-induced association between ITK and SLP-76, recruitment and transphosphorylation of ITK, actin polarization at the T-cell contact site, and expression of Th2 cytokines PMID: 20457812
Expression of patient-derived ITK in mice induces highly malignant peripheral T cell lymphomas. PMID: 20439541
Data show that tacrolimus modified the expression levels of Foxp3-regulated T cell receptor signal related-genes, PTPN22 and Itk, in Treg cells. PMID: 19714314
Itk catalytic activity is inhibited by the peptidyl prolyl isomerase activity of cyclophilin A (CypA). PMID: 11830645
In this review, the pathways are discussed by which Itk might impact the differentiation of T helper (Th) cells. PMID: 16931156
Itk forms dimers in the membrane and that receptors that recruit Itk do so to specific membrane regions PMID: 17060314
Vav phosphorylation correlates with calcium flux and Itk phosphorylation during mitogenic CD28 signalaing in Jurkat cells PMID: 17237383
Active signals from transgenic Tec kinase (ITK) regulate the development of memory-like CD8+ T cells with innate function. PMID: 17724684
Itk autophosphorylation on Y180 within the SH3 domain occurs exclusively via an intramolecular, in cis mechanism PMID: 17897671
In transgenic mice specifically lacking Itk kinase activity, active kinase signaling is required for control of T helper (Th)2 cell responses and development of allergic asthma. PMID: 18322190
These data suggest that inhibition of ITK blocks HIV infection by affecting multiple steps of HIV replication. PMID: 18443296
Analysis of SNPs in TCR pathway genes revealed that a haplotype that covers a major part of the coding sequence of ITK is a risk factor for seasonal allergic rhinitis. PMID: 19222422
ITK deficiency as a result of a R335W missense mutation, causes a novel immunodeficiency syndrome that leads to a fatal inadequate immune response to Epstein-Barr virus. PMID: 19425169
The authors identify the residues on the surface of the Itk SH2 domain responsible for substrate docking and show that this SH2 surface mediates autophosphorylation in the full-length Itk molecule. PMID: 19523959
Findings identify ITK-SYK as an active, transforming FTK and suggest ITK-SYK as a rational therapeutic target for t(5;9)(q33;q22)-positive lymphomas. PMID: 19535334
these results provide an initial step in understanding the biological role of Itk-TFII-I signaling in T-cell function. PMID: 19701889
The Tec family kinase Itk exists as a folded monomer in vivo. PMID: 19717557
Studies of Itk deletion mutants indicate that the amino acids in the N-terminal 152 residues and proline-rich domains enhance catalysis by affecting turnover rate rather than by substrate binding. PMID: 11437596

Hide All

Involvement in disease

Lymphoproliferative syndrome 1 (LPFS1)

Subcellular Location

Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, TEC subfamily

Tissue Specificity

T-cell lines and natural killer cell lines.

Database Links

HGNC: 6171

OMIM: 186973

KEGG: hsa:3702

STRING: 9606.ENSP00000398655

UniGene: Hs.558348

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Code	CSB-RA954682A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA954682A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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ITK Recombinant Monoclonal Antibody

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