Recombinant Human Tyrosine-protein kinase SYK(SYK)

Code CSB-YP022998HU
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Source Yeast
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Code CSB-EP022998HU
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Source E.coli
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Code CSB-EP022998HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022998HU
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Source Baculovirus
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Code CSB-MP022998HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names SYK
Uniprot No. P43405
Alternative Names EC 2.7.10.2; kinase Syk; KSYK; KSYK_HUMAN; p72-Syk; p72syk; Spleen tyrosine kinase; Syk; Tyrosine protein kinase SYK; Tyrosine-protein kinase SYK
Species Homo sapiens (Human)
Expression Region 1-635
Target Protein Sequence MASSGMADSA NHLPFFFGNI TREEAEDYLV QGGMSDGLYL LRQSRNYLGG FALSVAHGRK AHHYTIEREL NGTYAIAGGR THASPADLCH YHSQESDGLV CLLKKPFNRP QGVQPKTGPF EDLKENLIRE YVKQTWNLQG QALEQAIISQ KPQLEKLIAT TAHEKMPWFH GKISREESEQ IVLIGSKTNG KFLIRARDNN GSYALCLLHE GKVLHYRIDK DKTGKLSIPE GKKFDTLWQL VEHYSYKADG LLRVLTVPCQ KIGTQGNVNF GGRPQLPGSH PATWSAGGII SRIKSYSFPK PGHRKSSPAQ GNRQESTVSF NPYEPELAPW AADKGPQREA LPMDTEVYES PYADPEEIRP KEVYLDRKLL TLEDKELGSG NFGTVKKGYY QMKKVVKTVA VKILKNEAND PALKDELLAE ANVMQQLDNP YIVRMIGICE AESWMLVMEM AELGPLNKYL QQNRHVKDKN IIELVHQVSM GMKYLEESNF VHRDLAARNV LLVTQHYAKI SDFGLSKALR ADENYYKAQT HGKWPVKWYA PECINYYKFS SKSDVWSFGV LMWEAFSYGQ KPYRGMKGSE VTAMLEKGER MGCPAGCPRE MYDLMNLCWT YDVENRPGFA AVELRLRNYY YDVVN
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Required for the stimulation of neutrophil phagocytosis by IL15. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response.
Gene References into Functions
  1. In this article, the authors have shown that Natural killer cells can reduce their functional role through downregulation of Syk and Zap70 kinases. PMID: 29263215
  2. DFNA5 variant is associated with tobacco- and HPV-mediated oral oncogenesis. PMID: 30091681
  3. This study identifies a novel signaling involving SYK/c-MYC/MALAT1 as a promising therapeutic target for the treatment of Ewing sarcoma. PMID: 28336564
  4. Comparison of the distributions of tau pTyr18 and double-phosphorylated Syk in the transgenic mouse brain and human hippocampus showed that the phosphorylation of tyrosine 18 in tau already occurs at an early stage of tauopathy and increases with the progression of neurodegeneration. Syk appears unlikely to be a major kinase that phosphorylates tyrosine 18 of tau at the early stage of tauopathy. PMID: 28919467
  5. Results show that Syk activation occurs following Abeta deposition and the formation of tau pathological species. As Syk activation also promotes Abeta formation and tau hyperphosphorylation, results suggest that Alzheimer's disease pathological lesions may be self-propagating via a Syk dependent mechanism highlighting Syk as an attractive therapeutic target for the treatment of Alzheimer's disease. PMID: 28877763
  6. study reports that neutrophils constitutively express various Src family isoforms as well as Syk, and that inhibition of these protein tyrosine kinases selectively hinders inflammatory cytokine generation by acting posttranscriptionally PMID: 28512645
  7. expressed by immune cells and keratinocytes in skin lesions of cutaneous lupus erythematosus patients PMID: 26910509
  8. Cholesterol crystals activate specific cell signalling pathways which drive the production of inflammatory cytokines and degradative enzymes known to contribute to disease initiation and progression and are dependent on activation of Syk and PI3K PMID: 27356299
  9. Syk-induced signals in bone marrow stromal cell lines are mediated by phospholipase C gamma1 (PLCgamma1) in osteogenesis and PLCgamma2 in adipogenesis. PMID: 28786489
  10. AKT and 14-3-3 proteins down-regulate the activity of several BCR-associated components, including BTK, BLNK and SYK and also inhibit SYK's interaction with Importin 7 PMID: 27381982
  11. Expression of SYK and its splice variants was significantly lower in tumors with MSI, and in KRAS wild type, BRAF mutant and PTEN mutant tumors PMID: 28957395
  12. combined use of butyrate and highly specific Syk inhibitor BAY61-3606 does not enhance differentiation and apoptosis of colonocytes. Instead, BAY completely abolishes butyrate-induced differentiation and apoptosis in a Syk- and ERK1/2-dependent manner. PMID: 27293079
  13. Study demonstrates that redundant complexes formed by the mutant SYK(Y3F) protein allow it to maintain signaling to receptor-proximal interaction partners, resulting in proper initiation of BCR-proximal signals. PMID: 28760774
  14. The study identified the criteria for the design of binders that specifically address either the Syk or the Zap-70 Tandem Src Homology 2 Domains, tSH2. While Syk tSH2 has a rather broad substrate scope, ZAP-70 tSH2 required a proximal arrangement of the phosphotyrosine ligands in defined strand orientation. PMID: 28767218
  15. SYK mediates the actions of EPO and GM-CSF and coordinates with TGF-beta in erythropoiesis. PMID: 28131718
  16. study demonstrates that high SYK expression is a common feature of CD21(low) B cells independent of the underlying disorder, and that this high expression is sufficient to drive constitutive phosphorylation of SYK and its immediate targets Bruton's tyrosine kinase and phospholipase Cgamma2 PMID: 28468967
  17. IgG (mAb)-opsonized, inactivated Francisella tularensis LVS enhances macrophage and dendritic cell IL-1beta responses in a TLR2- and FcgammaR-dependent fashion. FcgammaR-mediated Syk activation leads to NLRP3 inflammasome-dependent IL-1beta production in macrophages. PMID: 27365531
  18. High SYK expression is associated with lymphangioleiomyomatosis. PMID: 28202529
  19. NKp65 utilizes a hemi-immunoreceptor tyrosine-based activation motif -like motif for cellular activation that requires Syk, although Syk appears not to be recruited to NKp65. PMID: 28082678
  20. Syk is a key regulator of Hoxa9/Meis1-driven acute myeloid leukemia. PMID: 28399410
  21. Data show that spleen tyrosine kinase (SYK) and proto-oncogene protein c-akt (AKT1) proteins were increased in the cytoplasm of the cells forming Mallory-Denk bodies. PMID: 28089901
  22. B cell receptor signaling component, SYK, caused PAX5 tyrosine phosphorylation in vitro and in cells. Transcriptional repression on the BLIMP1 promoter by PAX5 was attenuated by this phosphorylation. PMID: 27181361
  23. Pharmacological inhibitors of SYK activity significantly reduced the engulfment of oxLDL microbeads in the presence of serum factors, but had little effect on IgG phagocytosis. PMID: 27510553
  24. our data strongly suggest that AQCA-mediated suppression of inflammatory responses could be managed by a direct interference of signaling cascades including IRAK and Syk, linked to the activation of NF-kappaB and AP-1. PMID: 27338330
  25. a potential link between the upregulation of Syk and VEGF-C expression and lung adenocarcinoma. PMID: 27461624
  26. The lack of Syk mRNA expression in lung cancer play an important role in angiogenesis. PMID: 27461628
  27. Overall novel mutations in SYK gene and in silico analysis revealed that these mutations are crucial and might be responsible for altered expression of SYK. PMID: 26889814
  28. this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells PMID: 26980390
  29. SYK, LYN and PTPN6 were markedly elevated in atherosclerotic plaques of carotid atherosclerosis patients. PMID: 26742467
  30. Syk inhibitor suppressed the phosphorylation of band 3 also preventing serine phosphorylation changes and hemolysis. PMID: 27034738
  31. SYK expression was down-regulated in laryngeal squamous cell carcinoma, which was closely correlated with cancer growth and lymph node metastasis. SYK up-regulation was able to inhibit the invasion and metastasis of LSCC. PMID: 26884848
  32. TNF activates Mule by inducing the dissociation of Mule from its inhibitor ARF. Inhibition of Mule phosphorylation by silencing Syk prevents this, thereby inhibiting Mule E3 ligase activity and TNF-induced JNK activation and cell death. PMID: 26212014
  33. Study establishes P-SYK as a critical biomarker in AML that identifies tumors sensitive to SYK inhibition, identifies an at-risk patient population, and allows for the monitoring of target inhibition during treatment. PMID: 26315286
  34. LMPs are important pro-apoptotic regulators for Rb cells through reduction of SYK expression. PMID: 26404525
  35. Data suggest that immune complexes (ICs) of FCGR3A-SYK play critical role in activation of CD4+ T-cells and thus in modulation of adaptive immunity; such ICs are present in blood from patients with systemic lupus erythematosus. PMID: 26582197
  36. Syk activation is prominent in infiltrating myeloid cells in human rapidly progressive glomerulonephritis PMID: 26251216
  37. Results suggest that the activated Syk-mediated TRAF6 pathway leads to aberrant activation of B cells in SLE. PMID: 25432781
  38. this study shows that the treatment of cells with inducers of stress granule formation leads to the recruitment of Syk to these protein-RNA complexes PMID: 26429917
  39. The differential requirements of ZAP70 and SYK during thymic development. PMID: 26187144
  40. these data support the translation of approaches targeting Syk with fostamatinib to the clinic for patients with relapsed and possibly even newly diagnosed Waldenstrom macroglobulinemia PMID: 25748087
  41. Syk is constitutively associated with TLR4. PMID: 25896065
  42. The SYK -803 A>T genotypes TA and TT are independent risk factors for colorectal cancer development in Han Chinese in Southern China PMID: 25921550
  43. Data indicate that MINCLE receptor is able to mediate the response to trehalose-6,6-dimycolate (TDM) dependent on SYK kinase and CARD9 protein. PMID: 26202982
  44. find that both Syk and LMP2A have an effect on ITGbeta4 cell surface expression PMID: 25531330
  45. Demonstrate role for CBL in the control of AXL/SYK/LYN network mediating resistance to nilotinib treatment in chronic myeloid leukemia cells. PMID: 25965880
  46. SYK is downstream of CYR61 and contributes to CYR61-mediated mitoxantrone resistance. The CYR61-SYK pathway represents a potential target for reducing stroma-induced chemoresistance PMID: 25974135
  47. Results show that paclitaxel-resistant cells expressed higher level of phospho-SYK targeting tubulins and microtubule-associated proteins. PMID: 26096845
  48. An inflammatory Syk-ERK signaling axis requires DENV immune complexes, because DENV-2 in the presence of serotype-matched anti-DENV-2 mAb, but not anti-DENV-1 mAb, activates Syk, ERK, and IL-1beta secretion. PMID: 26032420
  49. PRT-060318 had a significant, albeit partial, inhibitory effect, thus suggesting that ROS induce NET formation in part via activation of Syk. PMID: 25277753
  50. The results reveal a Syk-dependent pathway of CpG-induced B cell stimulation, which is initiated at the plasma membrane and seems to be an upstream requirement for endosomal TLR9-driven B cell proliferation and differentiation. PMID: 25543269

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Subcellular Location Cell membrane. Cytoplasm, cytosol.
Protein Families Protein kinase superfamily, Tyr protein kinase family, SYK/ZAP-70 subfamily
Tissue Specificity Widely expressed in hematopoietic cells (at protein level). Expressed in neutrophils (at protein level). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.
Database Links

HGNC: 11491

OMIM: 600085

KEGG: hsa:6850

STRING: 9606.ENSP00000364898

UniGene: Hs.371720

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