Recombinant Human Tyrosine-protein phosphatase non-receptor type 3 (PTPN3), partial

Code CSB-YP019040HU
MSDS
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Source Yeast
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Code CSB-EP019040HU
MSDS
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Source E.coli
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Code CSB-EP019040HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019040HU
MSDS
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Source Baculovirus
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Code CSB-MP019040HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PTPN3
Uniprot No.
Alternative Names
cytoskeletal-associated protein tyrosine phosphatase; protein tyrosine phosphatase H1; protein tyrosine phosphatase non-receptor type 3; Protein-tyrosine phosphatase H1; PTN3_HUMAN; PTP-H1; PTPH1; PTPN3; Tyrosine-protein phosphatase non-receptor type 3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.
Gene References into Functions
  1. High expression of PTPN3 was significantly associated with unfavorable prognosis of GBM. Moreover, in GBM cell lines, PTPN3 promoted cell proliferation and invasion, and the PTP common inhibitor pervanadate suppressed GBM proliferation and invasion. PMID: 30348936
  2. PTPN3 promotes tumorigenicity, stemness and drug resistance in ovarian cancer. PMID: 27833130
  3. Results indicate that protein tyrosine phosphatase non-receptor type 3 (PTPN3) may act as a tumor suppressor in lung cancer through its modulation of epidermal growth factor receptor (EGFR) signaling. PMID: 25263444
  4. analysis of how allosteric regulation of p38gamma and PTPN3 involves a PDZ domain-modulated complex formation PMID: 25314968
  5. These studies thus identified PTPH1 as a novel ER phosphatase and further demonstrate a therapeutic potential of enhancing breast cancer sensitivity to antiestrogens through dephosphorylating ER by PTPH1. PMID: 24227889
  6. Activating mutations in and high expression levels of PTPN3 were associated with tumor recurrence in cholangiocarcinoma. PMID: 24503127
  7. p38gamma Mitogen-activated protein kinase signals through phosphorylating its phosphatase PTPH1 in regulating ras protein oncogenesis and stress response. PMID: 22730326
  8. The fusion transcript of ALK and PTPN3 identified resulted from translocation of a part of ALK gene into the third intron of PTPN3. Analysis of the transcript sequence indicates that no protein with any enzymatic activity is produced. PMID: 22334442
  9. PTPH1 stimulated breast cancer growth through regulating vitamin D receptor expression. PTPH1 was overexpressed in primary breast cancer and levels of its protein expression positively correlated with clinical metastasis. PMID: 21119599
  10. PTPH1 plays a role in Ras-dependent malignant growth by a mechanism depending on its p38gamma-binding activity. Ras increases p38gamma and PTPH1 expression and there is a coupling of increased p38gamma and PTPH1 protein expression in colon cancer. PMID: 20332238
  11. regulates TACE (tnf-alpha convertase} PMID: 12207026
  12. first reported demonstration that protein tyrosine phosphatase H1(PTPH1) is capable of interacting with and dephosphorylating T cell receptor zeta subunit PMID: 14672952
  13. PTPH1 binds to the PDZ-domain binding motif of the cardiac voltage-gated sodium channel Na(v)1 and regulates the activity of Na(v)1. PMID: 16930557
  14. Degradation of PTPN3 by HPV-16 E6 requires E6AP, the proteasome, and an interaction between the carboxy terminus of E6 and the PDZ domain of PTPN3. PMID: 17166906
  15. phosphatase activity and FERM domain of PTPN3 are essential for its suppression of HBV gene expression. PMID: 17588219
  16. PTP-H1 has a role in modulating GHR signaling and systemic growth through insulin-like growth factor 1 secretion PMID: 17921143
  17. Data suggest that reducing the level of PTPH1 may contribute to the oncogenic activity of high-risk genital E6 proteins. PMID: 17947517

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class subfamily
Database Links

HGNC: 9655

OMIM: 176877

KEGG: hsa:5774

STRING: 9606.ENSP00000363667

UniGene: Hs.436429

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