Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

RAD23A

Uniprot No.

P54725

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

hHR 23A; hHR23A; HR23A; MGC111083; RAD 23a; RAD23 homolog A (S. cerevisiae); RAD23 homolog A; RAD23 yeast homolog A; RAD23A; RD23A_HUMAN; UV excision repair protein RAD23; UV excision repair protein RAD23 homolog A

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-363aa

Target Protein Sequence

MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

66.6kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-363 constitute the expression domain of recombinant Human RAD23A. The theoretical molecular weight of the RAD23A protein is 66.6 kDa. This RAD23A recombinant protein is manufactured in e.coli. The RAD23A coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant RAD23A protein in following stages of expression and purification.

UV excision repair protein RAD23 homolog A (RAD23A) is a multifunctional protein involved in nucleotide excision repair (NER), a DNA repair mechanism that removes damaged nucleotides caused by UV radiation and other genotoxic agents. RAD23A acts as a shuttle protein that participates in the recognition and recruitment of damaged DNA sites during the initial steps of NER. It forms a complex with the Xeroderma Pigmentosum Group C (XPC) protein, aiding in the detection of DNA lesions. Additionally, RAD23A is involved in the delivery of ubiquitinated substrates to the proteasome for degradation. Studies on RAD23A contribute to the understanding of NER and the coordination of DNA repair processes. Aberrations in DNA repair mechanisms, including RAD23A function, are implicated in cancer development. Research explores the potential role of RAD23A in tumorigenesis. RAD23A's involvement in delivering ubiquitinated substrates to the proteasome connects it to cellular protein quality control and degradation pathways.

Target Background

Function

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.; Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.; (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.

Gene References into Functions

Data indicate that HR23A protein-depleted cells exhibit enhanced autophagy when treated with DNA-damaging agents. PMID: 27613096
HR23A role in DNA reapair, in protein degradation and stability, tumorigenesis and neurodegenerative disorders [review] PMID: 27771451
hHR23A associates with Chk1 through its ubiquitin-associated domains, and knockdown of hHR23A increases and stabilizes the protein level of Chk1 and its phosphorylation at S347. PMID: 26296656
Data indicate that phosphorylation provides a mechanism to regulate Rad23/proteasome interaction. PMID: 25311859
Here, we show that hHR23A utilizes both the UBA2 and XPCB domains to form a stable complex with Vpr, linking Vpr directly to cellular DNA repair pathways and their probable exploitation by the virus. PMID: 24318982
this study identified RAD23A as a novel negative regulator of RIG-I/MDA5 mediated anti-virus response. PMID: 23357418
Determined is the three-dimensional structure of its ubiquitin-like (UbL) domain by X-ray crystallography. PMID: 21047872
Vpr promotes hHR23A-mediated protein-ubiquitination, and down-regulation of hHR23A using RNAi significantly reduced viral replication in non-proliferating MAGI-CCR5 cells and primary macrophages PMID: 20614012
involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination PMID: 11788722
structures of the UBA domains of HHR23A reveal a conserved hydrophobic surface for protein-protein interactions PMID: 12079361
the solution structures of the HHR23A Ubl domain PMID: 12970176
Adopts a closed conformation and binds to the proteasomal subunit S5a thereby changing its own conformation. PMID: 14557549
hHR23 binds to polyubiquitylated p53 via its carboxyl-terminal ubiquitin-associated (Uba) domain shielding p53 from deubiquitylation PMID: 14645509
Data suggest that the UBL domain of HHR23A negatively regulates polyubiquitin/UBA interactions and identify leucine 8 of ubiquitin as an important determinant of chain recognition. PMID: 15321727
hHR23A regulates the function of xeroderma pigmentosum C by its association with the nucleotide excision repair activator p53 PMID: 16105547
Ufd4, the E3 component of the UFD pathway, is involved in controlling the degradation of Rad4, and Ufd4 and Rad23 exhibit a synthetic inhibitory effect on Rad4 degradation PMID: 16430867
hHR23B thus plays a critical role in the activation and function of p53 after specific genotoxic exposures. PMID: 16924240
hHR23a and hPLIC2 interact via UBL/UBA domain interactions PMID: 17098253
Pyramidal crystals of the UbL domain of hHR23A were diffracted to beyond 2 A resolution and the structure was solved by molecular replacement. PMID: 19724136

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Subcellular Location

Nucleus.

Protein Families

RAD23 family

Database Links

HGNC: 9812

OMIM: 600061

KEGG: hsa:5886

STRING: 9606.ENSP00000467024

UniGene: Hs.643267

Code	CSB-EP019259HU
Abbreviation	Recombinant Human RAD23A protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019259HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RAD23A. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019259HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RAD23A.
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Recombinant Human UV excision repair protein RAD23 homolog A (RAD23A)

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