Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
hHR 23A; hHR23A; HR23A; MGC111083; RAD 23a; RAD23 homolog A (S. cerevisiae); RAD23 homolog A; RAD23 yeast homolog A; RAD23A; RD23A_HUMAN; UV excision repair protein RAD23; UV excision repair protein RAD23 homolog A
Species
Homo sapiens (Human)
Expression Region
1-363aa
Target Protein Sequence
MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-363 constitute the expression domain of recombinant Human RAD23A. The theoretical molecular weight of the RAD23A protein is 66.6 kDa. This RAD23A recombinant protein is manufactured in e.coli. The RAD23A coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant RAD23A protein in following stages of expression and purification.
UV excision repair protein RAD23 homolog A (RAD23A) is a multifunctional protein involved in nucleotide excision repair (NER), a DNA repair mechanism that removes damaged nucleotides caused by UV radiation and other genotoxic agents. RAD23A acts as a shuttle protein that participates in the recognition and recruitment of damaged DNA sites during the initial steps of NER. It forms a complex with the Xeroderma Pigmentosum Group C (XPC) protein, aiding in the detection of DNA lesions. Additionally, RAD23A is involved in the delivery of ubiquitinated substrates to the proteasome for degradation. Studies on RAD23A contribute to the understanding of NER and the coordination of DNA repair processes. Aberrations in DNA repair mechanisms, including RAD23A function, are implicated in cancer development. Research explores the potential role of RAD23A in tumorigenesis. RAD23A's involvement in delivering ubiquitinated substrates to the proteasome connects it to cellular protein quality control and degradation pathways.