Recombinant Human Ubiquitin carboxyl-terminal hydrolase 3 (USP3)

Code CSB-YP896756HU
MSDS
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Source Yeast
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Code CSB-EP896756HU
MSDS
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Source E.coli
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Code CSB-EP896756HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896756HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP896756HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
USP3
Uniprot No.
Alternative Names
Deubiquitinating enzyme 3; EC 3.1.2.15; SIH003; Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin specific peptidase 3; Ubiquitin specific processing protease 3; Ubiquitin Specific Protease 3; Ubiquitin thioesterase 3; Ubiquitin thiolesterase 3; Ubiquitin-specific-processing protease 3; UBP; UBP3_HUMAN; Usp3
Species
Homo sapiens (Human)
Expression Region
1-520
Target Protein Sequence
MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG RYVNGHAKKH YEDAQVPLTN HKKSEKQDKV QHTVCMDCSS YSTYCYRCDD FVVNDTKLGL VQKVREHLQN LENSAFTADR HKKRKLLENS TLNSKLLKVN GSTTAICATG LRNLGNTCFM NAILQSLSNI EQFCCYFKEL PAVELRNGKT AGRRTYHTRS QGDNNVSLVE EFRKTLCALW QGSQTAFSPE SLFYVVWKIM PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS KRSKNQENGP VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI QKLPKVLCLH LKRFHWTAYL RNKVDTYVEF PLRGLDMKCY LLEPENSGPE SCLYDLAAVV VHHGSGVGSG HYTAYATHEG RWFHFNDSTV TLTDEETVVK AKAYILFYVE HQAKAGSDKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Gene References into Functions
  1. Data found that USP3 was overexpressed in gastric cancer (GC) tissues and cells. USP3 overexpression can be a useful biomarker for predicting the outcomes of GC patients. Moreover, USP3 was shown to influence cell proliferation by regulating cell cycle control and metastasis-related proteins. In vivo experiments showed that USP3 promoted GC tumor growth and metastasis. PMID: 30168892
  2. Depletion of USP3 lead to accelerated degradation of p53 in normal cells thereby enhanced cell proliferation and transformation. Reconstitution of wildtype USP3, but not the USP3 C168S mutant, restored the stability of p53 protein and inhibited cell proliferation and transformation. PMID: 28807825
  3. Taken together, the results suggested that USP3 is a negative regulator of ubiquitination signaling, counteracting RNF168- and RNF8-mediated ubiquitination. PMID: 24196443
  4. the role of UCP3 gene in functional status and survival at old age. PMID: 22743239
  5. Observational study, meta-analysis, and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20445134
  6. Ubiquitin-specific protease 3 (USP3) is a deubiquitinating enzyme for monoubiquitinated histones H2A and H2B; USP3 dynamically associates with chromatin and deubiquitinates H2A/H2B in vivo. PMID: 17980597

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Subcellular Location
Nucleus. Note=Localizes preferentially with monoubiquitinated H2A to chromatin.
Protein Families
Peptidase C19 family, USP3 subfamily
Tissue Specificity
Expressed in all tissues examined, with strongest expression in pancreas.
Database Links

HGNC: 12626

OMIM: 604728

KEGG: hsa:9960

STRING: 9606.ENSP00000369681

UniGene: Hs.458499

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