Recombinant Human Ubiquitin-fold modifier-conjugating enzyme 1 (UFC1)

Code CSB-YP897483HU
MSDS
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Source Yeast
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Code CSB-EP897483HU
MSDS
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Source E.coli
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Code CSB-EP897483HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897483HU
MSDS
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Source Baculovirus
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Code CSB-MP897483HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UFC1
Uniprot No.
Alternative Names
CGI-126; HSPC155; Ubiquitin fold modifier conjugating enzyme 1; Ubiquitin-fold modifier-conjugating enzyme 1; UFC1; UFC1_HUMAN; Ufm1 conjugating enzyme 1; Ufm1-conjugating enzyme 1
Species
Homo sapiens (Human)
Expression Region
1-167
Target Protein Sequence
MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E1-like enzyme which specifically catalyzes the second step in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
Gene References into Functions
  1. that UFC1 regulates survival of Osteoarthritis chondrocytes through physically association with miR-34a PMID: 27529373
  2. Autoantibodies binding to Ufc1 were found in 19 of 29 patients (66%) with active mycobacterial infections. PMID: 25213431
  3. Uba5 residues 364-404 were demonstrated to be necessary for the transthiolation of Ufm1 to Ufc1, and Uba5 381-404 was identified to be the minimal region for Ufc1 recognition PMID: 25084390
  4. UFC1 interact with the mRNA stabilizing protein HuR to increase levels of beta-catenin in hepatocellular carcinoma. PMID: 25449213
  5. NCAM140 interacts with ufc1 and its trafficking and endocytosis is upregulated in the presense of Ufm1. PMID: 24726913
  6. The structure of human Ufc1 was solved by using both NMR spectroscopy and X-ray crystallography PMID: 19101823
  7. Identification of a novel enzyme called UFC1 for Ufm1-conjugating enzyme 1. PMID: 15071506

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Protein Families
Ubiquitin-conjugating enzyme family, UFC1 subfamily
Database Links

HGNC: 26941

OMIM: 610554

KEGG: hsa:51506

STRING: 9606.ENSP00000356982

UniGene: Hs.301412

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