Recombinant Human Urea transporter 2 (SLC14A2), partial

Code CSB-YP620898HU
MSDS
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Source Yeast
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Code CSB-EP620898HU
MSDS
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Source E.coli
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Code CSB-EP620898HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP620898HU
MSDS
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Source Baculovirus
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Code CSB-MP620898HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC14A2
Uniprot No.
Alternative Names
FLJ16167; hUT-A6; HUT2; kidney; MGC119566; MGC119567; Slc14a2; Solute carrier family 14 (urea transporter), member 2; Solute carrier family 14 member 2; Urea transporter 2; Urea transporter; Urea transporter, kidney; UT-A2; UT2; UT2_HUMAN; UTA; UTR
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism.
Gene References into Functions
  1. The urea transporter subtypes, UT-A1 and UT-B1, were expressed in the skin basal cell layer and exocrine sweat glands. The abundance of UT-A1 and UT-B1 in uremic sweat glands was significantly increased in UP, while the expression of AQP5 was decreased. PMID: 29279852
  2. UT2 is a negative regulator shared across STAT3 and mTORC2 signaling cascades, functioning as a tumor suppressor in hematologic malignancies driven by those pathways. PMID: 26927669
  3. Studies indicate that the urea transporter SLC14a2, the UT-A group, was originally isolated from kidney inner medulla. PMID: 25298338
  4. Studies indicate that osmotic diuresis, where urea concentrations are low, can prompt an increase in urea transporter abundance. PMID: 25298340
  5. Studies indicate that the cell signaling pathway for vasopressin-mediated urea transporters phosphorylation and activity involves two cAMP-dependent signaling pathways. PMID: 25298341
  6. HUT2 gene may play a certain role in developing metabolic syndrome and its related traits in Asian population. PMID: 20797394
  7. a novel UT-A urea transporter; the transcript contains a novel 129-bp exon, exon 5a, which, as a result of alternative splicing, introduces a unique 19-aa segment and a stop codon. PMID: 15189812
  8. results provide new evidences that suggest the involvement of AQP9 and UT-A in the urea excretion mechanism across human term placenta from mother to fetus in physiological conditions PMID: 16480766
  9. This review summarizes the published work that has accrued on the structure and regulation of these genes. PMID: 17264986
  10. polymorphisms in the SLC14A2 gene can predict the antihypertensive efficacy of nifedipine GITS (gastrointestinal therapeutic system) PMID: 17344938

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Subcellular Location
Apical cell membrane; Multi-pass membrane protein.
Protein Families
Urea transporter family
Tissue Specificity
Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney.
Database Links

HGNC: 10919

OMIM: 601611

KEGG: hsa:8170

STRING: 9606.ENSP00000255226

UniGene: Hs.710927

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