Recombinant Human Wee1-like protein kinase 2 (WEE2)

Code CSB-YP026085HU
MSDS
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Source Yeast
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Code CSB-EP026085HU
MSDS
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Source E.coli
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Code CSB-EP026085HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026085HU
MSDS
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Source Baculovirus
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Code CSB-MP026085HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WEE2
Uniprot No.
Alternative Names
hCG_39453; WEE1 homolog 2 (S. pombe); Wee1-like protein kinase 1B; Wee1-like protein kinase 2; WEE1B; Wee1B kinase; Wee2; WEE2_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-567
Target Protein Sequence
MDDKDIDKEL RQKLNFSYCE ETEIEGQKKV EESREASSQT PEKGEVQDSE AKGTPPWTPL SNVHELDTSS EKDKESPDQI LRTPVSHPLK CPETPAQPDS RSKLLPSDSP STPKTMLSRL VISPTGKLPS RGPKHLKLTP APLKDEMTSL ALVNINPFTP ESYKKLFLQS GGKRKIRGDL EEAGPEEGKG GLPAKRCVLR ETNMASRYEK EFLEVEKIGV GEFGTVYKCI KRLDGCVYAI KRSMKTFTEL SNENSALHEV YAHAVLGHHP HVVRYYSSWA EDDHMIIQNE YCNGGSLQAA ISENTKSGNH FEEPKLKDIL LQISLGLNYI HNSSMVHLDI KPSNIFICHK MQSESSGVIE EVENEADWFL SANVMYKIGD LGHATSINKP KVEEGDSRFL ANEILQEDYR HLPKADIFAL GLTIAVAAGA ESLPTNGAAW HHIRKGNFPD VPQELSESFS SLLKNMIQPD AEQRPSAAAL ARNTVLRPSL GKTEELQQQL NLEKFKTATL ERELREAQQA QSPQGYTHHG DTGVSGTHTG SRSTKRLVGG KSARSSSFTS GEREPLH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
Gene References into Functions
  1. homozygous mutations in WEE2 that are responsible for fertilization failure in humans, were identified. PMID: 29606300
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
Tissue Specificity
Expressed in oocytes (at protein level). May also be expressed in testis.
Database Links

HGNC: 19684

OMIM: 614084

KEGG: hsa:494551

STRING: 9606.ENSP00000380675

UniGene: Hs.657927

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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