Recombinant Human Zinc finger protein 300 (ZNF300)

Code CSB-YP026653HU
MSDS
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Source Yeast
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Code CSB-EP026653HU
MSDS
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Source E.coli
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Code CSB-EP026653HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026653HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP026653HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ZNF300
Uniprot No.
Alternative Names
DKFZp686B24204; Kruppel like zinc finger protein; OTTHUMP00000160554; OTTHUMP00000206798; Zinc finger protein 300; ZN300_HUMAN; ZNF300
Species
Homo sapiens (Human)
Expression Region
1-604
Target Protein Sequence
MMKSQGLVSF KDVAVDFTQE EWQQLDPSQR TLYRDVMLEN YSHLVSMGYP VSKPDVISKL EQGEEPWIIK GDISNWIYPD EYQADGRQDR KSNLHNSQSC ILGTVSFHHK ILKGVTRDGS LCSILKVCQG DGQLQRFLEN QDKLFRQVTF VNSKTVTEAS GHKYNPLGKI FQECIETDIS IQRFHKYDAF KKNLKPNIDL PSCYKSNSRK KPDQSFGGGK SSSQSEPNSN LEKIHNGVIP FDDNQCGNVF RNTQSLIQYQ NVETKEKSCV CVTCGKAFAK KSQLIVHQRI HTGKKPYDCG ACGKAFSEKF HLVVHQRTHT GEKPYDCSEC GKAFSQKSSL IIHQRVHTGE KPYECSECGK AFSQKSPLII HQRIHTGEKP YECRECGKAF SQKSQLIIHH RAHTGEKPYE CTECGKAFCE KSHLIIHKRI HTGEKPYKCA QCEEAFSRKT ELITHQLVHT GEKPYECTEC GKTFSRKSQL IIHQRTHTGE KPYKCSECGK AFCQKSHLIG HQRIHTGEKP YICTECGKAF SQKSHLPGHQ RIHTGEKPYI CAECGKAFSQ KSDLVLHQRI HTGERPYQCA ICGKAFIQKS QLTVHQRIHT VVKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has a transcriptional repressor activity.
Gene References into Functions
  1. Our findings further support ZNF300P1 as frequently methylated in ovarian cancer and reveal a novel function for ZNF300P1 lincRNA expression PMID: 24393131
  2. A novel function for ZNF300 in tumour development was identified that may uniquely link inflammation and NF-kappa B to tumourigenesis in humans but not in mice. PMID: 21777376
  3. Data show that co-expression of ZNF300 and Egr1 lead to enhanced IL-2Rbeta promoter activity, and suggest that ZNF300 is another regulator of the human IL-2Rbeta promoter. PMID: 20585888
  4. ZNF300 was activated by PU.1 and suggested that the regulation may be involved in the progression of leukemia development and hematopoietic differentiation. PMID: 20471086
  5. ZNF-300 and novel splice variant ZNF300-B are both expressed highly in human testis. PMID: 17541838
  6. Results show that the ZNF300 gene encodes a 68-kDa nuclear protein that binds DNA in a sequence-specific manner. PMID: 18350257

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Subcellular Location
Nucleus.
Protein Families
Krueppel C2H2-type zinc-finger protein family
Tissue Specificity
Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis.
Database Links

HGNC: 13091

OMIM: 612429

KEGG: hsa:91975

STRING: 9606.ENSP00000397178

UniGene: Hs.134885

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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