Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ZP3

Uniprot No.

P21754

Research Area

Developmental Biology

Alternative Names

HUMZP3; Processed zona pellucida sperm-binding protein 3; Sperm receptor; zona pellucida glycoprotein 3 (sperm receptor); Zona pellucida glycoprotein 3; zona pellucida glycoprotein 3A (sperm receptor); Zona pellucida glycoprotein ZP3; Zona pellucida protein C; Zona pellucida sperm binding protein 3 precursor; Zp-3; ZP3 372; ZP3 424; ZP3 474; ZP3; ZP3_HUMAN; ZP3A; ZP3A/ZP3B; ZP3B; ZPC

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

23-387aa

Target Protein Sequence

QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

56.6kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human ZP3 was expressed with the amino acid range of 23-387. This ZP3 protein is expected to have a theoretical molecular weight of 56.6 kDa. Expression of this ZP3 protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of ZP3, which enables a simple process of detecting and purifying the ZP3 recombinant protein in the following steps.

The human zona pellucida sperm-binding protein 3 (ZP3) is a glycoprotein that plays a crucial role in the process of fertilization. ZP3 is a component of the zona pellucida, an extracellular matrix surrounding the oocyte. During fertilization, ZP3 serves as a sperm receptor, facilitating the binding and recognition of spermatozoa to the zona pellucida. This interaction triggers the acrosome reaction in sperm, enabling them to penetrate the zona pellucida and reach the oocyte for fertilization. ZP3 is essential for species-specific fertilization, as it ensures that only sperm of the same species can bind to and fertilize the egg. Additionally, ZP3 has been studied for its potential applications in contraceptive vaccine development, as antibodies against ZP3 can block sperm-egg interaction, providing a contraceptive effect.

Target Background

Function

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Gene References into Functions

The zona pellucida-3 (ZP3) protein plays a pivotal role in oocyte and gamete development. PMID: 30341457
The oocyte ZP3 expression was the main predictor of the fertilization capacity. PMID: 28204536
Missense Mutation in ZP3 gene is associated with Empty Follicle Syndrome and Female Infertility. PMID: 28886344
Epididymal CRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3. PMID: 24334245
observed sequence variations in exons of ZP3 gene in women with infertility of unknown origin who exhibit abnormal zona pellucida; sperm-ovum interactions appear relatively normal in these patients [CASE REPORTS] PMID: 22889493
Eggs expressing glycoprotein huZP3, derived from transgenic mice, bind murine but not human sperm, implying that huZP3 acquires the same O-glycans as native mZP3. PMID: 14673092
human and mouse ZP3 proteins are quite similar, and alternative explanations of taxon-specific sperm binding warrant exploration PMID: 15379548
Binding sites for recombinant zona pellucida C (ZPC) glycoprotein are located both at the N- and C-terminus of proacrosin PMID: 15950651
Peptides rhuZP3a22 approximately 176 and rhuZP3b177 approximately 348 have a role similar to human ZP3. The mechanism of the response to the peptides involves influx of calcium, the G protein pathway, and a T-type calcium channel. PMID: 17192598
may have a role in the development of primordial follicle before zona pellucida formation PMID: 18502569
induces acrosome reactions which are protein kinase-C, protein tyrosine kinase, T-type Ca2+ channels, and extracellular Ca2+ dependent PMID: 18667750
a significant decrease in acrosomal exocytosis mediated by both recombinant human ZP3 (p<0.005) and ZP4 (p<0.005) was observed in presence of the immune sera PMID: 19004505
The functional activity of human ZP3 resides in its C-terminal domain. PMID: 19246320

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Involvement in disease

Oocyte maturation defect 3 (OOMD3)

Subcellular Location

[Processed zona pellucida sperm-binding protein 3]: Zona pellucida.; Cell membrane; Single-pass type I membrane protein.

Protein Families

ZP domain family, ZPC subfamily

Tissue Specificity

Expressed in oocytes (at protein level).

Database Links

HGNC: 13189

OMIM: 182889

KEGG: hsa:7784

STRING: 9606.ENSP00000378326

UniGene: Hs.656137

Code	CSB-EP027121HU
Abbreviation	Recombinant Human ZP3 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP027121HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ZP3. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP027121HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ZP3.
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Recombinant Human Zona pellucida sperm-binding protein 3 (ZP3), partial

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