Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Developmental Biology
Alternative Names
HUMZP3; Processed zona pellucida sperm-binding protein 3; Sperm receptor; zona pellucida glycoprotein 3 (sperm receptor); Zona pellucida glycoprotein 3; zona pellucida glycoprotein 3A (sperm receptor); Zona pellucida glycoprotein ZP3; Zona pellucida protein C; Zona pellucida sperm binding protein 3 precursor; Zp-3; ZP3 372; ZP3 424; ZP3 474; ZP3; ZP3_HUMAN; ZP3A; ZP3A/ZP3B; ZP3B; ZPC
Species
Homo sapiens (Human)
Expression Region
23-387aa
Target Protein Sequence
QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSV
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human ZP3 was expressed with the amino acid range of 23-387. This ZP3 protein is expected to have a theoretical molecular weight of 56.6 kDa. Expression of this ZP3 protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of ZP3, which enables a simple process of detecting and purifying the ZP3 recombinant protein in the following steps.
The human zona pellucida sperm-binding protein 3 (ZP3) is a glycoprotein that plays a crucial role in the process of fertilization. ZP3 is a component of the zona pellucida, an extracellular matrix surrounding the oocyte. During fertilization, ZP3 serves as a sperm receptor, facilitating the binding and recognition of spermatozoa to the zona pellucida. This interaction triggers the acrosome reaction in sperm, enabling them to penetrate the zona pellucida and reach the oocyte for fertilization. ZP3 is essential for species-specific fertilization, as it ensures that only sperm of the same species can bind to and fertilize the egg. Additionally, ZP3 has been studied for its potential applications in contraceptive vaccine development, as antibodies against ZP3 can block sperm-egg interaction, providing a contraceptive effect.
