Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
L3Protease; EC 3.4.22.39; Adenain; Adenovirus protease; AVP; Adenovirus proteinase; Endoprotease
Species
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Expression Region
1-204aa
Target Protein Sequence
MGSSEQELKAIVKDLGCGPYFLGTYDKRFPGFVSPHKLACAIVNTAGRETGGVHWMAFAWNPHSKTCYLFEPFGFSDQRLKQVYQFEYESLLRRSAIASSPDRCITLEKSTQSVQGPNSAACGLFCCMFLHAFANWPQTPMDHNPTMNLITGVPNSMLNSPQVQPTLRRNQEQLYSFLERHSPYFRSHSAQIRSATSFCHLKNM
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The N-terminal 10xHis tag and C-terminal Myc tag was fused to the gene fragment corresponding to the 1-204aa of the human adenovirus C serotype 5 L3 protein and then was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant human adenovirus C serotype 5 L3 protein. Its purity is higher than 90%. This recombinant L3 protein showed an apparent molecular weight of about 30 kDa under SDS-PAGE condition.
Cleaves viral precursor proteins including pTP, pIIIa, pVI, pVII, pVIII, and pX could lead to the production of mature virions. Compared to the unmature virions, mature virions show a weakened organization, which further enhances the subsequent uncoating. The particle lacks infectivity and is unable to uncoat as there is no maturation. In the cytoplasm, L3Protease may participate in the cytoskeleton destruction and cleaves host cell cytoskeletal keratins K7 and K18.