Code | CSB-EP356968HML |
Abbreviation | Recombinant Human papillomavirus type 16 E4 protein |
MSDS | |
Size | US$388 |
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The expression of the recombinant Human papillomavirus type 16 Protein E4 involves the construction of a plasmid encoding the Human papillomavirus type 16 Protein E4 (1-92aa). This resulting plasmid is introduced into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. After that, cultured cells are lysed. The subsequent step includes purifying the recombinant Human papillomavirus type 16 Protein E4 through affinity purification from the cell lysate and assessing the purity of the protein via SDS-PAGE. The purity of the recombinant Protein E4 is greater than 85%.
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KEGG: vg:1489076