Name: Recombinant Oldenlandia affinis Kalata-B1 (OAK1)
Brand: CUSABIO
SKU: 349763OAZ

Product Details

Purity

>85% (SDS-PAGE)

Target Names

OAK1

Uniprot No.

P56254

Alternative Names

OAK1; Kalata-B1

Species

Oldenlandia affinis

Expression Region

89-117

Target Protein Sequence

GL PVCGETCVGG TCNTPGCTCS WPVCTRN

Protein Length

Cytoplasmic domain

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

From what I understood, the lab only expresses the expression region (89-117aa)? When produced in yeast, is it produced in Pichia pastoris? Also, this peptide is known to be a cyclotide, the circular structure is highly relevant for its bio-activity. The enzyme asparagine endoproteinase is essential for cyclization of the peptide. We would like to order this peptide to do some functional tests. I was wondering how are you making sure you are producing a mature circular peptide and that cyclization is taken place?

Yes, currentlly, we have only produced 89-117aa region from which the signal peptide and propeptide are removed.
Regarding the CSB-YP349763OAZ protein expressed by yeast, we use Pichia pastoris, and the commonly used strains are gs115 strain and X33 strain.
We are sorry that since it is a semi-custom product and hasn't been developed and produced yet, for the protein structure and activity, we can't test it, so we can't guarantee that it will meet the customer's expectations.
Looking at the structural studies in the literature, most of the sequences are constructed without signal peptide and N-terminal propeptide: https://www.uniprot.org/uniprotkb/P56254/entry#structure
In yeast expression, we use secretory vector, so the yeast protein is expressed in supernatant, which is very likely to be active. But customers still need to verify the activity themselves, because we do not guarantee the activity.

Target Background

Function

Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.

Protein Families

Cyclotide family, Moebius subfamily

Tissue Specificity

Leaves and stems. Lower in roots.

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Code	CSB-YP349763OAZ
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP349763OAZ-B
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Code	CSB-BP349763OAZ
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Code	CSB-MP349763OAZ
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Recombinant Oldenlandia affinis Kalata-B1 (OAK1)

Product Details

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Customer Reviews and Q&A

Target Background

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