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Recombinant Lactobacillus delbrueckii subsp. bulgaricus Glyceraldehyde-3-phosphate dehydrogenase (gap)

Recombinant Lactobacillus delbrueckii subsp. bulgaricus Glyceraldehyde-3-phosphate dehydrogenase (gap)

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Code	CSB-EP009232LMM
Abbreviation	Recombinant Lactobacillus delbrueckii subsp. bulgaricus gap protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

gap

Uniprot No.

O32755

Research Area

Others

Species

Lactobacillus delbrueckii subsp. bulgaricus

Source

E.coli

Expression Region

1-338aa

Target Protein Sequence

MTVKIGINGFGRIGRLAFRRIMDLGEETKDIEVVAINDLTTPAMLAHLLKYDSTHGTFDHEVSATEDSLVVDGKKYRVYAEPQAQNIPWVKNDGVDFVLECTGFYTSKAKSQAHLDAGAKRVLISAPAGNDLKTIVYSVNQDTLTADDTIVSAGSCTTNSLAPMANALNKEFGIQVGTMTTIHAYTATQKVLDGPDRGNNFRNARAAAENIIPHSTGAAKAIGLVLPELNGKLDGHAQRVPVKDGSETELVTILDKKVTAEEVNAAMKKYESPSFAYNADQIVSTDVLGMTAGSIFDPTQTQVITAGDKQLVKTVAWYDNEYSFTCQMVRTLLHFATL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

44.0 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant Lactobacillus delbrueckii subsp. bulgaricus Glyceraldehyde-3-phosphate dehydrogenase (gap) is created by inserting the gene encoding the full-length Lactobacillus delbrueckii subsp. bulgaricus gap protein (1-338aa) and the N-terminal 10xHis-tag and C-terminal Myc-tag gene into a plasmid and transferring the recombinant plasmid into E.coli cells for protein production. The recombinant gap protein is isolated and purified from the cell lysate. Its purity is greater than 90% measured by SDS-PAGE. On the gel, the recombinant gap protein appears as a band with a molecular weight of approximately 45 kDa.

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Target Background

Function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Subcellular Location

Cytoplasm.

Protein Families

Glyceraldehyde-3-phosphate dehydrogenase family

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