Recombinant Macaca fascicularis Coagulation factor IX (F9) (Active)

Factor IX plays a central role in the intrinsic coagulation cascade, and this cynomolgus macaque ortholog provides a non-human primate model for hemostasis research and preclinical therapeutic evaluation. The protein demonstrates robust catalytic function with a specific activity exceeding 50 pmol/min/µg against the fluorogenic peptide substrate MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)NH2, supporting its use in enzymatic activity assays, kinetic parameter determination (Km, Vmax, kcat), and inhibitor screening with IC50 quantification. Expressed as the full-length mature protein spanning residues 29–461 with a C-terminal 10×His tag, this design preserves the active site architecture required for accurate substrate specificity profiling and drug candidate evaluation. Purity greater than 95% by SDS-PAGE and endotoxin levels below 1.0 EU/µg satisfy the quality thresholds commonly required for enzyme kinetics studies and provide a suitable basis for structural investigations including crystallography.