Recombinant Macaca fascicularis Dipeptidase 3(DPEP3) (Active)

DPEP3 belongs to the membrane-bound dipeptidase family and has emerged as a target of interest in cancer metabolism research, where its role in peptide catabolism may influence tumor microenvironment dynamics. This full-length mature protein (aa 36–463) demonstrates specific antibody-binding activity with an EC50 of 7.817–8.936 ng/mL in functional ELISA, providing quantitative evidence that the recombinant construct retains native epitope presentation and supports its use as a positive control in immunoassays and as a reagent for inhibitor screening campaigns where consistent target recognition is essential. The C-terminal His tag placement preserves the N-terminal catalytic domain architecture, enabling researchers to conduct enzymatic activity assays, kinetic parameter analysis (Km, Vmax, kcat), and substrate specificity profiling without steric interference at the active site. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the quality thresholds commonly required for accurate kinetic measurements, IC50 determination in drug candidate evaluation, and structural studies including crystallography where contaminant-free samples are critical for interpretable diffraction data.