Purity

≥ 95% as determined by SDS-PAGE.
≥ 90% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ITGAV&ITGB6(CSB-MP6541MOV) at 2 μg/mL can bind Macaca mulatta TGFB1. The EC50 is 8.899-14.82 ng/mL.

Target Names

TGFB1

Uniprot No.

F7HCV5

Alternative Names

TGFB1

Species

Macaca mulatta (Rhesus macaque)

Source

Mammalian cell

Expression Region

30-390aa(C33S)

Target Protein Sequence

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSKDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Mol. Weight

67.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal hFc1-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

TGF-β1 orchestrates immune suppression, fibrosis, and epithelial-mesenchymal transition across diverse tissue contexts, making its receptor-binding properties central to mechanistic studies of these processes. This rhesus macaque ortholog, spanning the complete mature protein sequence (aa 30–390) with a stabilizing C33S substitution, demonstrates functional integrin αVβ6 binding with an EC50 of 8.9–14.8 ng/mL in ELISA, providing quantitative evidence of receptor engagement suitable for competition assays, receptor-ligand interaction studies by surface plasmon resonance, and antibody epitope mapping experiments. Mammalian cell expression preserves the post-translational modifications required for proper folding and biological activity, supporting its use in cell-based assays including fibroblast proliferation, myofibroblast differentiation, and immune cell suppression models. Purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg align with standards expected in sensitive primary cell culture systems and functional immunology experiments.

Code	CSB-MP6752MOW(A4)(M)
Abbreviation	Recombinant Rhesus macaque TGFB1 protein (C33S) (Active)
MSDS	MSDS Datasheet
Size	$190
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ITGAV&ITGB6(CSB-MP6541MOV) at 2 μg/ml can bind Macaca mulatta TGFB1. The EC₅₀ is 8.899-14.82 ng/mL. Biological Activity Assay The purity of TGFB1 was greater than 90% as determined by SEC-HPLC
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Recombinant Macaca mulatta Transforming growth factor beta (TGFB1) (C33S) (Active)

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