Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Neuroscience
Alternative Names
Pon1; Pon; Serum paraoxonase/arylesterase 1; PON 1; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
Species
Mus musculus (Mouse)
Expression Region
2-355aa
Target Protein Sequence
AKLLALTLVGLVLALYKNHRSSYQTRLNAFREVTPVELPNCNLVKGIETGAEDLEILPNGLTFFSTGLKYPGIKSFDPSKPGKILLMDLNKKEPAVSELEIIGNTLDISSFNPHGISTFTDEDNTVYLLVVNHPDSSSTVEVFKFQEEERSLLHLKTITHELLPSINDIAAIGPESFYATNDHYFADPYLRSWEMYLGLSWSNVVYYSPDKVQVVAEGFDFANGIGISLDGKYVYIAELLAHKIHVYEKHANWTLTPLKVLNFDTLVDNISVDPVTGDLWVGCHPNGMRIFFYDAENPPGSEVLRIQNILSEDPKITVVYAENGTVLQGTTVASVYKGKLLIGTVFHKALYCDL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Mouse Pon1 contains amino acids 2-355. The calculated molecular weight for this Pon1 protein is 39.4 kDa. Expression of this Pon1 protein is conducted in e.coli. The Tag-Free was smoothly integrated into the coding gene of Pon1, which enables a simple process of detecting and purifying the Pon1 recombinant protein in the following steps.
The mouse serum paraoxonase/arylesterase 1 (Pon1) plays a crucial role in detoxification and antioxidation processes. Its primary function involves the hydrolysis and detoxification of organophosphates, commonly found in pesticides, safeguarding the body against their toxic effects. Additionally, Pon1 exhibits arylesterase activity, contributing to the breakdown of various substrates, including lipid peroxides, thereby acting as an antioxidant. The enzyme's association with high-density lipoprotein (HDL) particles suggests its involvement in cardiovascular health, where it may play a protective role against atherosclerosis. Pon1's diverse enzymatic activities make it a subject of interest in research fields ranging from toxicology and environmental health, focusing on its role in detoxification, to cardiovascular research, exploring its potential impact on lipid metabolism and atherosclerotic processes.
