Recombinant Mouse Acetyl-CoA carboxylase 1 (Acaca), partial

Code CSB-YP729191MO
MSDS
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Source Yeast
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Code CSB-EP729191MO
MSDS
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Source E.coli
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Code CSB-EP729191MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP729191MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP729191MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Acaca
Uniprot No.
Alternative Names
Acaca; Acac; Gm738Acetyl-CoA carboxylase 1; ACC1; EC 6.4.1.2; ACC-alpha; Acetyl-CoA carboxylase 265
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Gene References into Functions
  1. the present studies report for the first time a role of ACC1 in suppressing breast cancer migration and invasion by an fatty acid synthesis-independent, but acetyl-CoA-dependent, impact on the epithelial-mesenchymal transition programs in breast tumor cells and its subsequent importance for tumor invasion and recurrence. PMID: 29056512
  2. madecassic acid was the active form of madecassoside in ameliorating colitis by restoring the Th17/Treg balance via regulating the PPARgamma/AMPK/ACC1 pathway. PMID: 28358365
  3. Studied a role for ACAC inactivation in meiotic resumption by testing the effect of two ACAC inhibitors, CP-640186 and Soraphen A, on mouse oocytes maintained in meiotic arrest in vitro. PMID: 26043180
  4. Results suggest an essential role for acetyl coenzyme A carboxylase 1 (ACC1)-mediated de novo lipogenesis as a regulator of CD8(+) T cell expansion. PMID: 24567531
  5. the stimulation of fatty acid oxidation by modulating the AMPK-ACC-CPT-1 pathway may be part of a protective mechanism against saturated free fatty acids that drive podocyte death. PMID: 24338821
  6. A biotin analog inhibits acetyl-CoA carboxylase activity and adipogenesis PMID: 11907024
  7. Expression of the isoenzyme of Acc1 is mediated by MyoD and MRF4 is differentially affected by retinoic acid receptor and retinoid X receptor. PMID: 12642900
  8. Mutant mice lacking acetyl-CoA carboxylase 1 are embryonically lethal. PMID: 16103361
  9. when fed fat-free diet for 10 days, there was significant up-regulation of PPARgamma and several enzymes in the lipogenic pathway in the liver of liver-specific ACC1 knockout mice compared with the WT mice PMID: 16717184
  10. description of a pathway in which Tribbles 3 (TRB3) stimulates lipolysis by triggering the degradation of acetyl-coenzyme A carboxylase (ACC) in adipose tissue; TRB3 promoted ACC ubiquitination through an association with the E3 ubiquitin ligase COP1 PMID: 16794074
  11. Hepatic de novo lipogenesis is present in liver-specific ACC1-deficient mice. PMID: 17210641
  12. Downregulation of ACC1 on osteoblastogenesis may be the cause for the osteopenia phenotype of FACC1 knockout bone homeostasis. PMID: 19805143

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Subcellular Location
Cytoplasm, cytosol.
Database Links
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