Recombinant Mouse Afadin- and alpha-actinin-binding protein (Ssx2ip)

Code CSB-YP823729MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP823729MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP823729MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP823729MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP823729MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ssx2ip
Uniprot No.
Alternative Names
Ssx2ipAfadin- and alpha-actinin-binding protein; ADIP; Afadin DIL domain-interacting protein
Species
Mus musculus (Mouse)
Expression Region
1-615
Target Protein Sequence
MGDWMTVTDP VLCTENKNLS QYTSETKMSP SSLYSQQVLC SSVPLSKNVH GVFGVFCTGE NIEQSISYLD QELTTFGFPS LYEESKSKEA KRELNIVAVL NCMNELLVLQ RKNLLAQESV ETQNLKLGSD MDHLQSCYAK LKEQLETSRR EMIGLQERDR QLQCKNRSLH QLLKNEKDEV QKLQNIIASR ATQYNHDVKR KEREYNKLKE RLHQLVMNKK DKNIAMDVLN YVGRADGKRG SWRTDKTEAR NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR ERAEDGTGTV AISDIEDDSG ELSRDSVWGL SCDTVREQLT NSIRKQWRIL KSHVEKLDNQ ASKVHSEGLN EEDVISRQDH EQETEKLELE IERCKEMIKA QQQLLQQQLA TTCDDDTTSL LRDCYLLEEK ERLKEEWTLF KEQKKNFERE RRSFTEAAIR LGLERKAFEE ERASWVKQQF LNMTNFDHQN SENVKLFSAF SGSSDPDNLI VHSRPRQKKL HSVANGVPAC TSKLTKSLPA SPSTSDFRQT HSCVSEHSSI SVLNITPEES KPSEVAREST DQKWSVQSRP SSREGCYSGC SSAFRSAHGD RDDLP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
Gene References into Functions
  1. The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone. PMID: 24356449
  2. The results indicate that ADIP plays an essential role in PDGF-induced cell movement by interacting with afadin and Vav2 and regulating the activation of Rac. PMID: 22027834
  3. localization at cell-cell adherens junctions PMID: 12446711
Subcellular Location
Cell junction, adherens junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body. Note=Not found at cell-matrix AJs.
Protein Families
ADIP family
Tissue Specificity
Widely expressed.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1