Recombinant Mouse BAG family molecular chaperone regulator 4 (Bag4)

Code CSB-YP814550MO
MSDS
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Source Yeast
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Code CSB-EP814550MO
MSDS
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Source E.coli
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Code CSB-EP814550MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP814550MO
MSDS
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Source Baculovirus
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Code CSB-MP814550MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bag4
Uniprot No.
Alternative Names
Bag4; SoddBAG family molecular chaperone regulator 4; BAG-4; Bcl-2-associated athanogene 4; Silencer of death domains
Species
Mus musculus (Mouse)
Expression Region
1-457
Target Protein Sequence
MSALRRSGYG PSDGPSYGRY YGPGGGDVPV HVPPPLYPPL RPEPPQPPVS WRGRGGAPAE TTWPGEGAGG DGYYPSGGAW AEASRAGGGH QEQPPYPGYN SNYWNSVRPR APYPGSYSVR PELQGQSLNS YANGAYGPPY PPGPGASTAS YSGAYYVPGY TQSNYSTEVP NTYRSPGNSP TPMSRWMYSQ QDCPTEAPPL RGQVPGYPAS QNPGMTLPHY PYGDGNRAVP QSGGTGRPQD DAWASSAYGM GARYPWPSAA PSAPSAGSLY MTESASPWPG NSSPQPPPSP PPQQPKDPSY SYNPSGQGLS RHSFPCSVHQ YESPGAVNND NSDLLDSQVQ YSAEPQLYGN ASSEHPSNQV PSNNLPEECF SSDEGTPPSI KKIIHVLEKV QFLEQEVEEF VGKKTDKAYW LLEEMLTKEL LELDSVETGG QDSVRQARKE AVCKIQAILE KLEKKGL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Prevents constitutive TNFRSF1A signaling. Negative regulator of PRKN translocation to damaged mitochondria.
Gene References into Functions
  1. Silencer of death domains (SODD) inhibits skeletal muscle and kidney enriched inositol 5-phosphatase (SKIP) and regulates phosphoinositide 3-kinase (PI3K)/Akt signaling to the actin cytoskeleton. PMID: 21712384
  2. SODD/BAG-4 is not only a silencer of TNFR1 but also a modulator of CD95 activity, regulating the balance of both death-promoting and -depressing signals PMID: 12706861
  3. Results suggest that silencer of death domains protein (SODD) is critical for the regulation of tumor necrosis factor signaling. PMID: 12748303
  4. BAG-4 functions as a negative regulator for apobec-1-mediated apoB mRNA editing PMID: 14559896
  5. The proapoptotic proteins Bak (Bcl-2 antagonistic killer) and Bax (Bcl-2-associated X protein) were depleted in livers from TCPOBOP-treated CAR+/+ mice. PMID: 16799968

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Subcellular Location
Cytoplasm.
Database Links
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