Product Details

Purity

>85% (SDS-PAGE)

Target Names

Batf

Uniprot No.

O35284

Alternative Names

BatfBasic leucine zipper transcriptional factor ATF-like; B-cell-activating transcription factor; B-ATF

Species

Mus musculus (Mouse)

Expression Region

1-125

Target Protein Sequence

MPHSSDSSDS SFSRSPPPGK QDSSDDVRKV QRREKNRIAA QKSRQRQTQK ADTLHLESED LEKQNAALRK EIKQLTEELK YFTSVLSSHE PLCSVLASGT PSPPEVVYSA HAFHQPHISS PRFQP

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.

Gene References into Functions

BATF plays a key role in the differentiation of T helper cell subsets and Ig class-switching. Apart from that we suggest an important function in mast cell development and in murine models of allergic asthma, it was observed that mice deficient for Batf are protected from the disease. Hence, the previous findings on BATF make it an interesting target for the treatment of Th cell-derived-cytokine-driven diseases. PMID: 26970726
repressed BATF expression contributes to impaired Treg functions caused by A384T mutation in Foxp3 PMID: 28778586
these findings reveal BATF as a central modulator of peripheral and humoral hallmarks of type-2 immunity and begin to elucidate a novel mechanism by which it regulates type-2 cytokine production through its modification of the Th2 locus control region. PMID: 27798167
Our studies uncovered the critical role of two transcription factors, IRF1 and BATF, in preparing the chromatin landscape for induction of the Tr1 gene network in response to IL-27 signaling, where BATF acted as a pioneer factor and prepared the genomic landscape for the binding of additional transcription factors that define the Tr1 lineage. PMID: 28166218
BATF/JUN-B and BATF/C-JUN complexes play important roles in OA cartilage destruction through regulating anabolic and catabolic gene expression in chondrocytes. PMID: 27147707
this study shows that the affinity of different enhancers for the BATF-IRF4 complex might underlie divergent signaling outcomes in response to various strengths of TCR signaling PMID: 28346410
BATF overexpression sufficiently rescues "unhelped" CD8 T cells. BATF and IRF4 cooperatively regulate Blimp-1 expression and effector T cell function . PMID: 26527008
These results indicate a positive role of Batf in promoting the generation of pro-allergic IL-4-producing T-follicular helper cells. PMID: 26278622
The transcription factors IRF4 and BATF are major regulators of antiviral cytotoxic T-cell immunity. PMID: 24531538
BATF is essential for initial commitment of naive T8 cells to effector development but becomes dispensable by 72h. This makes possible a study of gene function in vivo in unperturbed cells of hematopoietic origin that are refractory to viral transduction. PMID: 25548173
amplifies CD8+ T cell TCR-dependent expression of transcription factors and inflammatory signals but restrains the expression of effector molecules PMID: 24584090
knock-out mice have reduced pathophysiologic signs of asthma, decreased IL-3 production and mast cells development PMID: 24290279
The activator protein 1 (AP1) family transcription factor BATF (B cell, activating transcription factor-like) was among the genes enriched in Th9 cells and was required for the expression of IL-9 and other Th9-associated genes. PMID: 24216482
BATF joins RORgammat as the molecular signature for all IL-17 producing cells in vivo. PMID: 23537103
A previously unknown function of early growth response gene (Egr)-2 in the control of T helper (Th)17 cytokine expression and Th17 differentiation by inhibition of Batf function. PMID: 23203924
BATF-JUN family protein complexes cooperate with IRF4 in binding to AP1-IRF composite elements in pre-activated CD4( ) T cells stimulated with IL-21 and in T(H)17 differentiated cells PMID: 22992523
Compensation among BATF factors is based on the shared capacity of their leucine zipper domains to interact with non-AP1 factors such as IRF4 and IRF8 to mediate cooperative gene activation. PMID: 22992524
BATF, together with c-Jun, transcriptionally inhibited expression of the nicotinamide adenine dinucleotide (NAD(+))-dependent deacetylase Sirt1 PMID: 21873234
Data show that BATF functions at multiple hierarchical levels in two cell types to globally regulate switched antibody responses in vivo. PMID: 21572431
Batf mediates the differentiation-inducing effects of Stat3 signaling in M1 cells and suggest that Batf may play a similar role in other blood cell lineages where alterations to the Jak-Stat pathway are hallmarks of disrupted development and disease. PMID: 21296860
PD-1 inhibits T cell function by upregulating BATF. PMID: 20890291
Loss of Batf disrupts multiple components of the lymphocyte communication network that are required for a robust immune response. PMID: 20421391
This study revealed that older BAFF transgenic mice exhibit an anxiety-like phenotype associated with brain inflammation. PMID: 20185032
Stat3 mediates cellular growth by modulating AP-1 activity through the induction of BATF PMID: 12444555
Fos/Jdp2/Batf locus is associated with SL3-3 mouse leukemia virus B cell lymphoma. PMID: 15913695
results demonstrate that the AP-1 protein BATF has a critical role in T(H)17 differentiation PMID: 19578362

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Subcellular Location

Nucleus. Cytoplasm. Note=Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells.

Protein Families

BZIP family

Tissue Specificity

Detected in postnatal and adult lymphoid tissues such as thymus, spleen and lymph nodes. In thymus most concentrated expression is found in the immediate cortical layer. Differentially expressed during T-cell development in thymus. Highly expressed in Th1

Database Links

KEGG: mmu:53314

STRING: 10090.ENSMUSP00000040706

UniGene: Mm.6672

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Recombinant Mouse Basic leucine zipper transcriptional factor ATF-like (Batf)

Product Details

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