Recombinant Mouse Bcl-2-binding component 3(Bbc3)

Code CSB-YP859563MO
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Source Yeast
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Code CSB-EP859563MO
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Source E.coli
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Code CSB-EP859563MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859563MO
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Source Baculovirus
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Code CSB-MP859563MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Bbc3
Uniprot No. Q99ML1
Alternative Names Bbc3; PumaBcl-2-binding component 3; p53 up-regulated modulator of apoptosis
Species Mus musculus (Mouse)
Expression Region 1-193
Target Protein Sequence MARARQEGSS PEPVEGLARD SPRPFPLGRL MPSAVSCSLC EPGLPAAPAA PALLPAAYLC APTAPPAVTA ALGGPRWPGG HRSRPRGPRP DGPQPSLSPA QQHLESPVPS APEALAGGPT QAAPGVRVEE EEWAREIGAQ LRRMADDLNA QYERRRQEEQ HRHRPSPWRV MYNLFMGLLP LPRDPGAPEM EPN
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
Gene References into Functions
  1. Expression from a luciferase reporter construct containing the 2 kb fragment of the promoter region of bcl-2-binding component 3 (PUMA) is enhanced by activation of the muscle regulatory transcription factor MyoD in both myoblasts and fibroblasts and diminishes by silencing of MyoD in myoblasts. E-Box Sequences at position - 857 of PUMA is the binding site for MyoD. PMID: 28918507
  2. Proapoptotic proteins BIM and PUMA are not critical for the reticulocyte apoptosis caused by loss of the pro-survival protein BCL-XL. PMID: 28682312
  3. The mitochondrial apoptotic pathway, activated by BH3-only proteins, BIM and PUMA, is essential for endoplasmic reticulum stress-induced cell death; DR5 as well as caspase-8 are not required for this process. PMID: 28409774
  4. Mir143-BBC3 has roles in microglial survival in the context of drug abuse PMID: 27464000
  5. In the mouse model of silicosis, Bbc3 knockout mice clearly exhibited decreased levels of autophagy and fibrosis progression. These results suggest that downregulation of BBC3 expression may become a novel therapeutic strategy for the treatment of silicosis. PMID: 28277537
  6. This study has demonstrated that methamphetamine-mediated pericytes migration involves PUMA up-regulation. PMID: 28408317
  7. Data show that Emu-Myc mice lacking both p21 and PUMA developed lymphoma at a rate considerably longer latency than Emu-Myc;p53(+/-)mice. PMID: 26640149
  8. Our data show a novel pathway of infection-induced apoptosis that enhances our understanding of the mechanism by which BH3-only proteins control apoptotic host cell death during Listeria infection. PMID: 27064265
  9. Overexpression of miR-29 or miR-24 is sufficient to inhibit the induction of Bim and Puma in young sympathetic neurons. PMID: 27797453
  10. loss of PUMA had no impact on the loss of platelets caused by loss of BCL-XL. It therefore remains to be established whether other BH3-only proteins play a critical role in induction of apoptosis in platelets or whether their death is controlled solely by the interactions between BCL-XL with BAK and BAX. PMID: 27221652
  11. PUMA is dispensable for glucose homeostasis in lean and obese mice, but it can affect leptin levels and food intake during obesity. PMID: 27033313
  12. MYSM1 is a critical negative regulator of p53 transcriptional programs in hematopoiesis, and its repression of Bbc3/PUMA expression is essential for multipotent progenitor survival, and partly contributes to maintaining hematopoietic stem cel function. PMID: 26768662
  13. Therapeutic response to non-genotoxic activation of p53 by Nutlin3a is driven by PUMA-mediated apoptosis in lymphoma cells. PMID: 26904937
  14. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. PMID: 26179069
  15. Beta-arrestin 2 promotes inflammation-induced intestinal epithelial apoptosis through endoplasmic reticulum stress/PUMA in colitis. PMID: 25354317
  16. Puma is the major mediator of virus-induced Bax/Bak activation and mitochondrial membrane permeabilization induction. PMID: 26030884
  17. Oxidative stress increases PUMA expression regulated by FoxO1 in follicular granulosa cells. PMID: 25425107
  18. Results indicate that PUMA is involved in the apoptosis of cerebral astrocytes upon ischemia/reperfusion injury PMID: 25451294
  19. by preventing the consumption of IL-15, Bim limits the role of Noxa and Puma in causing the death of effector cells with less memory potential. PMID: 25124553
  20. These results demonstrate that antagonism between PUMA and MCL-1 constitutes the major axis of control of hematopoietic stem cell survival. PMID: 25847014
  21. caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells. PMID: 25356864
  22. Stable knockdown of Bim or PUMA expression prevented cell toxicity induced by loss of Keap1. PMID: 24769730
  23. Loss of Bbc3 results in prolonged fertility. PMID: 24859845
  24. c-Jun transactivates Puma gene expression to promote osteoarthritis. PMID: 24566851
  25. Data demonstrate that high concentrations of glucose induce endoplasmic reticulum and oxidative stress, which causes pancreatic beta cell death mediated by Bim and Puma. PMID: 24625983
  26. PUMA mediates endoplasmic reticulum stress-induced mucosal epithelial apoptosis through the mitochondrial apoptotic pathway in portal hypertensive gastropathy. PMID: 24625987
  27. Pdx1 gene expression was knocked down in mouse MIN6 insulinoma cells resulting in apoptotic cell death that was found to be associated with increased expression of BH3-only molecules Bim and Puma. PMID: 24658302
  28. Slug promotes survival during metastasis through suppression of Puma-mediated apoptosis. PMID: 24830722
  29. The data if this study demonstrated that Puma is pivotal for oligodendroglial cell death induced by different cell death stimuli. PMID: 23922240
  30. these results provide strong evidence that Puma, like Bim, Noxa, and tBid, is able to act as a direct Bak activator. PMID: 24265320
  31. PUMA deficiency leads to a better survival rate associated with reduced DNA damage and fewer chromosomal aberrations in induced pluripotent stem cells, whereas loss of p21 or p53 results in an opposite outcome. PMID: 23873265
  32. Induction of senescence was only impaired in cells from the p21-/- puma-/- noxa-/- mice but abrogated in cells from the p53-/- mice. PMID: 23665218
  33. Microglia-derived TNF-alpha induces apoptosis in neural precursor cells via transcriptional activation of the Bcl-2 family member Puma. PMID: 23492769
  34. amino acid (AA) starvation elevates PUMA, an effect that is blocked by extracellular acidity. PMID: 23261451
  35. PUMA protein regulates autophagy, Erk activation, and intracellular calcium level. PMID: 23122957
  36. a novel and critical link between the AKT, GSK3beta and JNK kinases and the regulation of Puma induction PMID: 23056511
  37. Transforming growth factor-beta directly induces p53-up-regulated modulator of apoptosis (PUMA) during the rapid induction of apoptosis in myc-driven B-cell lymphomas PMID: 23243310
  38. Our study demonstrates a role for PUMA in the death of retinal ganglion cells. PMID: 23365256
  39. Data show that PUMA and NOXA are critical for DNA damage-induced, TAp63-mediated primordial follicle oocyte apoptosis. PMID: 23000175
  40. TLR4 is expressed on intestinal stem cells where it regulates their proliferation and apoptosis through activation of PUMA. PMID: 22955282
  41. Data show that Puma cooperates with Bim to impose a thymic-deletion checkpoint to peripheral self-antigens and cement the notion that defects in apoptosis alone are sufficient to cause autoimmune disease. PMID: 22960223
  42. Context-dependent enhancement of induced pluripotent stem cell reprogramming by silencing Puma PMID: 22311782
  43. identify Puma and Bim as key mediators of DNA damage-induced bone marrow failure and provide mechanistic insight into how BH3-only proteins trigger cell death. PMID: 22015606
  44. Cytokine receptor signaling activates an IKK-dependent phosphorylation of PUMA to prevent cell death. PMID: 21997190
  45. Deletion of Puma improves stem- and progenitor-cell function. PMID: 22138576
  46. Regulatory T cells can suppress the proliferation of Bim-deficient/Puma-deficient conventional T cells in a manner largely indistinguishable from wild-type conventional T cells. PMID: 21949016
  47. in response to DNA-damage, Noxa efficiently induces apoptosis by "release" of Puma from Mcl-1. PMID: 21945433
  48. Puma is a major regulator of memory B lymphocyte survival and therefore a key molecule in the control of the immune response. PMID: 21868573
  49. In mice and humans, colon inflammation induces apoptosis of intestinal epithelial cells via p53-dependent and - independent mechanisms; PUMA also activates an intrinsic apoptosis pathway associated with colitis. PMID: 21699775
  50. Bim and Puma expression is induced by endogenous ROS in a SIRT1-dependent manner. PMID: 21083429

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Subcellular Location Mitochondrion.
Protein Families Bcl-2 family
Database Links

KEGG: mmu:170770

STRING: 10090.ENSMUSP00000002152

UniGene: Mm.7660

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