Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Pla2g5; Calcium-dependent phospholipase A2; EC 3.1.1.4; Group V phospholipase A2; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5
Species
Mus musculus (Mouse)
Expression Region
21-137aa
Target Protein Sequence
GLLELKSMIEKVTGKNAFKNYGFYGCYCGWGGRGTPKDGTDWCCQMHDRCYGQLEEKDCAIRTQSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLYQYYPNFLC
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Mouse Calcium-dependent phospholipase A2 (Pla2g5) recombinant protein was produced in E.coli, where the gene sequence encoding Mouse Pla2g5 (21-137aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this Pla2g5 protein was greater than 90% by SDS-PAGE.
Pla2g5 is an enzyme that primarily functions by catalyzing the hydrolysis of phospholipids, producing free fatty acids and soluble phospholipids. This process plays a crucial role in the metabolism of cell membranes, cell signal transduction, and inflammatory responses. Pla2g5 is typically expressed in various tissues and cell types, including the liver, kidneys, gastrointestinal tract, spleen, and white blood cells. Its specific biological roles in the organism include maintaining the integrity of cell membranes, regulating cell signal transduction, and participating in inflammatory responses.
Some members of the Pla2g5 enzyme family have been found to be associated with inflammatory responses and diseases. Pla2g5 may play a role in modulating the inflammatory process, as the reaction it catalyzes leads to the release of phospholipids, triggering cell membrane rupture and lipid-mediated inflammatory responses, among other effects.