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The synthesis of this Recombinant Mouse Cfi protein depends on the utilization of recombinant DNA technology. DNA sequences that encoded the Cfi protein could be inserted into a vector and introduced into an expression host, Mammalian cell, where it could be easily expressed in and purified from. The expression of this Cfi protein was at 19-603aa. N-terminal 10xHis tag & C-terminal Myc tag was fused with this protein. The purity is 85%+ determined by SDS-PAGE.
Cfi (also known as If) is gene encoding a protein named complement factor I (Cfi or C3B/C4B inactivator). The protein encoded by this gene is a protein of the complement system, first isolated in 1966 in guinea pig serum. It is cleaved into three subcomponents by proteolytic cleavage, including C3b, iC3b and C4b. This protein mainly takes parts in two biological processes, complement activation signaling pathway and innate immune response with the scavenger receptor and serine-type endopeptidase activity.
|Purity||Greater than 85% as determined by SDS-PAGE.|
|Research Area||Epigenetics and Nuclear Signaling|
Cfi; IfComplement factor I; EC 126.96.36.199; C3B/C4B inactivator) [Cleaved into: Complement factor I heavy chain; Complement factor I light chain]
|Species||Mus musculus (Mouse)|
|Target Protein Sequence||KNTPASGQPQEDLVEQKCLLKNYTHHSCDKVFCQPWQKCIEGTCACKLPYQCPKAGTPVCATNGRGYPTYCHLKSFECLHPEIKFSNNGTCTAEEKFNVSLIYGSTDTEGIVQVKLVDQDEKMFICKNSWSTVEANVACFDLGFPLGVRDIQGRFNIPVNHKINSTECLHVRCQGVETSLAECTFTKKSSKAPHGLAGVVCYTQDADFPTSQSFQCVNGKRIPQEKACDGVNDCGDQSDELCCKGCRGQAFLCKSGVCIPNQRKCNGEVDCITGEDESGCEEDKKNKIHKGLARSDQGGETEIETEETEMLTPDMDTERKRIKSLLPKLSCGVKRNTHIRRKRVVGGKPAEMGDYPWQVAIKDGDRITCGGIYIGGCWILTAAHCVRPSRYRNYQVWTSLLDWLKPNSQLAVQGVSRVVVHEKYNGATYQNDIALVEMKKHPGKKECELINSVPACVPWSPYLFQPNDRCIISGWGREKDNQKVYSLRWGEVDLIGNCSRFYPGRYYEKEMQCAGTSDGSIDACKGDSGGPLVCKDVNNVTYVWGIVSWGENCGKPEFPGVYTRVASYFDWISYYVGRPLVSQYNV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Mol. Weight||69.8 kDa
|Protein Length||Full Length of Mature Protein|
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.|
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.
|Gene References into Functions||
|Subcellular Location||Secreted, extracellular space.|
|Protein Families||Peptidase S1 family|
|Tissue Specificity||Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes.|