Mouse Complement factor I(CFI) ELISA kit

Code CSB-EL005279MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
complement factor I
Alternative Names
Cfi ELISA Kit; IfComplement factor I ELISA Kit; EC 3.4.21.45 ELISA Kit; C3B/C4B inactivator) [Cleaved into: Complement factor I heavy chain; Complement factor I light chain] ELISA Kit
Abbreviation
CFI
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 97
Range % 93-100
1:2000 Average % 85
Range % 81-89
1:4000 Average % 96
Range % 93-99
1:8000 Average % 87
Range % 85-90
Recovery
The recovery of mouse CFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-106
EDTA plasma (n=4) 85 81-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
50 2.230 2.301 2.266 2.161
25 2.020 1.987 2.004 1.899
12.5 1.601 1.700 1.651 1.546
6.25 1.085 1.125 1.105 1.000
3.12 0.596 0.568 0.582 0.477
1.56 0.357 0.361 0.359 0.254
0.78 0.205 0.212 0.209 0.104
0 0.102 0.108 0.105  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CFI ELISA Kit was designed for the quantitative measurement of Mouse CFI protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

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Target Background

Function
(From Uniprot)
Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.
Gene References into Functions
  1. Factor I-mediated generation of activated C3 fragments in the circulation is a critical determinant for the development of MPGN2 associated with factor H deficiency. PMID: 18202746
Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
Tissue Specificity
Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes.
Database Links
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