Recombinant Mouse Cyclin-dependent kinase 4 inhibitor D (Cdkn2d)

Code CSB-YP730656MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP730656MO
MSDS
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Source E.coli
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Code CSB-EP730656MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730656MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP730656MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Cdkn2d; Cyclin-dependent kinase 4 inhibitor D; p19-INK4d
Species
Mus musculus (Mouse)
Expression Region
1-166
Target Protein Sequence
MLLEEVCVGD RLSGAAARGD VQEVRRLLHR ELVHPDALNR FGKTALQVMM FGSPAVALEL LKQGASPNVQ DASGTSPVHD AARTGFLDTL KVLVEHGADV NALDSTGSLP IHLAIREGHS SVVSFLAPES DLHHRDASGL TPLELARQRG AQNLMDILQG HMMIPM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Interacts strongly with CDK4 and CDK6 and inhibits them.
Gene References into Functions
  1. Rescue from early-onset hearing loss in a mouse model lacking the cyclin-dependent kinase inhibitor p19Ink4d. PMID: 26962681
  2. p19 INK4d controls hematopoietic stem cells in a cell-autonomous manner during genotoxic stress and through the microenvironment during aging PMID: 25458892
  3. Deletion of p19(Ink4d) (p19), a cyclin-dependent kinase CDK inhibitor gene, in mice results in spontaneous development of tumors in multiple organs and tissues. PMID: 24687853
  4. We propose that p19INK4d participates in the cellular mechanisms that trigger senescence by contributing to chromatin compaction PMID: 24667034
  5. an asymmetrical distribution pattern for Cdkn2d transcripts in 2-cell embryos. PMID: 22071115
  6. Ectopic expression of RUVBL2 decreases the levels of ARF, whereas knockdown of RUVBL2 results in a marked increase in ARF levels. In addition, RUVBL2 down-regulates the levels of p53 in an ARF-dependent manner. PMID: 22285491
  7. A significant proportion of immortalised cell cultures apparently had neither a p53 mutation nor loss of p19/ARF. PMID: 20378935
  8. Deletion of Ink4d alone is sufficient to disrupt the maintenance of the post-mitotic state of sensory hair cells in post-natal mice. In Ink4d-/- animals, hair cells re-enter the cell cycle and undergo apoptosis, resulting in progressive hearing loss. PMID: 12717441
  9. Cranial neural crest-derived mesenchymal proliferation is regulated by Msx1-mediated p19(INK4d) expression during odontogenesis. PMID: 12941628
  10. These studies indicate that a reduction in Ink4 family function by the loss of p19(Ink4d) is sufficient to induce mild reproductive defects in male mice with a mixed genetic background, but not in the C57BL/6 genetic background. PMID: 17393423
  11. p19(INK4D) knockdown led to a moderate increase (31.7% +/- 5%) in the mean ploidy of megakaryocytes suggesting a role of p19(INK4D) in the endomitotic arrest. PMID: 18276842

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
CDKN2 cyclin-dependent kinase inhibitor family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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