Recombinant Mouse Cytohesin-3 (Cyth3)

Code CSB-YP006469MO
MSDS
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Source Yeast
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Code CSB-EP006469MO
MSDS
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Source E.coli
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Code CSB-EP006469MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006469MO
MSDS
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Source Baculovirus
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Code CSB-MP006469MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cyth3
Uniprot No.
Alternative Names
Cyth3; Grp1; Pscd3Cytohesin-3; ARF nucleotide-binding site opener 3; Protein ARNO3; General receptor of phosphoinositides 1; Grp1; PH; SEC7 and coiled-coil domain-containing protein 3; CLM3; SEC7 homolog C; mSec7-3
Species
Mus musculus (Mouse)
Expression Region
1-399
Target Protein Sequence
MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL GERDDFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGRVKT WKRRWFILTD NCLYYFEYTT DKEPRGIIPL ENLSIREVED PRKPNCFELY NPSHKGQVIK ACKTEADGRV VEGNHVVYRI SAPSPEEKEE WMKSIKASIS RDPFYDMLAT RKRRIANKK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays a role in the epithelial polarization.
Gene References into Functions
  1. Structural dynamics control allosteric activation of Grp1 and ARF6. PMID: 29276036
  2. Mutation of a key residue (K273A) within the canonical phosphatidylinositol phosphate - binding site of Grp1 significantly reduced the free energy of phosphatidylinositol phosphate binding. PMID: 26977543
  3. Grp1 localizes at the beta1 integrin-positive intracellular compartment, and is involved in HGF-dependent Arf6 activation, beta1 integrin recycling and tumour angiogenesis and growth in mice. PMID: 26239146
  4. These observations suggest that Grp1 family guanine nucleotide exchange factors are autoregulated by mechanisms that depend on plasma membrane recruitment for activation. PMID: 18042453
Subcellular Location
Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, adherens junction. Cell junction, tight junction. Note=Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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