Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Fam20c

Uniprot No.

Q5MJS3

Research Area

Others

Alternative Names

Fam20c; Dmp4; Kiaa4081Extracellular serine/threonine protein kinase FAM20C; EC 2.7.11.1; Dentin matrix protein 4; DMP-4; Golgi-enriched fraction casein kinase; GEF-CK

Species

Mus musculus (Mouse)

Source

Baculovirus

Expression Region

88-579aa

Target Protein Sequence

DFSSDPASNLTSHSLEKLPSAAEPVDHAPRGQEPRSPPPRDPAHRPLLRDPGPRPRVPPPGPSGDGSLLAKLFEHPLYQGAVPPLTEDDVLFNVNSDIRFNPKAAENPDWPHEGAEGAEFLPTGEAAVNLYPNWLKFHIGINRYELYSRHNPAIDALLRDLGSQKITSVAMKSGGTQLKLIMTFQNYGQALFKPMKQTREQETPPDFFYFSDYERHNAEIAAFHLDRILDFRRVPPVAGRMINMTKEIRDVTRDKKLWRTFFVSPANNICFYGECSYYCSTEHALCGRPDQIEGSLAAFLPDLSLAKRKTWRNPWRRSYHKRKKAEWEVDPDYCEEVKQTPPYDSGHRILDIMDMTVFDFLMGNMDRHHYETFEKFGNETFIIHLDNGRGFGKYSHDELSILAPLHQCCRIRRSTYLRLQLLAKEEHKLSLLMAESLQHDKVAPVLYQLHLEALDRRLRIVLQAVRDCVEKDGLSSVVEDDLATEHRASTER
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

60.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Mouse Fam20c was expressed with the amino acid range of 88-579. This Fam20c protein is expected to have a theoretical molecular weight of 60.4 kDa. This Fam20c protein is produced using baculovirus expression system. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of Fam20c, making it easier to detect and purify the Fam20c recombinant protein in the later stages of expression and purification.

Fam20c (Extracellular serine/threonine protein kinase FAM20C) is a protein of significant interest. Researchers are eager to explore its crucial role in bone and tooth development, especially its regulatory mechanisms in mineralization processes and enamel formation. Additionally, Fam20c plays an important role in cell signaling and phosphorylation modification, prompting scientists to contemplate its potential impact on diseases such as skeletal deformities and abnormal tooth formation. These studies strive to unveil the molecular mechanisms of Fam20c, providing new theoretical foundations for the future treatment and prevention of bone and tooth-related disorders.

Target Background

Function

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth. Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome. Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity. Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1. In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion.

Gene References into Functions

that Fam20c indeed regulates cell behaviors and cell signaling pathway in a cell-autonomous manner PMID: 29337188
family with sequence similarity member 20C is the primary, but not the only, kinase for the SIBLINGs PMID: 26324849
Fam20A potentiates Fam20C kinase activity and promotes the phosphorylation of enamel matrix proteins in vitro. PMID: 25789606
Loss of Fam20C function leads to periodontal disease in mice PMID: 25479552
Successful generation of Fam20C-GFP transgenic mice will provide a unique model for studying Fam20C gene expression and the biological function of this gene during odontogenesis and osteogenesis PMID: 25537657
we have successfully generated the immortalized mouse floxed Fam20c dental papilla mesenchymal and osteoblast cell lines. PMID: 25833681
These results indicated that the downregulation of Dmp1 may not directly associate with, or significantly contribute to the bone and dentin defects in the Fam20C-cKO mice. PMID: 24874551
that FAM20C is not a constituent of the enamel extracellular matrix and functions intracellularly within ameloblasts. PMID: 23703840
FAM20C is a molecule essential to amelogenesis, its inactivation in the dental epithelium does not significantly affect dentinogenesis. PMID: 24026952
FAM20C is essential to the differentiation and mineralization of dental tissues through the regulation of molecules critical to the differentiation of tooth-formative cells. PMID: 22936805
Our findings indicate that FAM20C is essential to the differentiation of osteoblasts/osteocytes and is involved in the regulation of phosphate homeostasis via the mediation of FGF23 PMID: 22615579
Fam20c null mice develop hypophosphatemic rickets and defective enamel and dentin PMID: 22732358
temporospatial expression profile of FAM20C PMID: 20644212
This study defines the causative role of FAM20C in this lethal osteosclerotic disorder and its crucial role in normal bone development. PMID: 17924334

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Subcellular Location

Secreted. Golgi apparatus.

Protein Families

FAM20 family

Tissue Specificity

Highly expressed in the tooth. No expression in the dental pulp. At the secretory stage of amelogenesis, it is detected in the matrix of the enamel, in the ameloblasts, and within the cells adjoining the stratum intermedium (a tissue layer analogous to th

Database Links

KEGG: mmu:80752

STRING: 10090.ENSMUSP00000026972

UniGene: Mm.320355

Code	CSB-BP702804MO
Abbreviation	Recombinant Mouse Fam20c protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Extracellular serine/threonine protein kinase FAM20C (Fam20c)

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