Recombinant Mouse Developmental pluripotency-associated protein 3 (Dppa3)

Code CSB-YP844422MO
MSDS
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Source Yeast
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Code CSB-EP844422MO
MSDS
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Source E.coli
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Code CSB-EP844422MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844422MO
MSDS
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Source Baculovirus
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Code CSB-MP844422MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dppa3
Uniprot No.
Alternative Names
Dppa3; Cap1p; Crg1; Pgc7; Developmental pluripotency-associated protein 3; Compaction-associated protein 1; Primordial germ cell protein 7
Species
Mus musculus (Mouse)
Expression Region
1-150
Target Protein Sequence
MEEPSEKVDP MKDPETPQKK DEEDALDDTD VLQPETLVKV MKKLTLNPGV KRSARRRSLR NRIAAVPVEN KSEKIRREVQ SAFPKRRVRT LLSVLKDPIA KMRRLVRIEQ RQKRLEGNEF ERDSEPFRCL CTFCHYQRWD PSENAKIGKN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.
Gene References into Functions
  1. The absence of the DPPA3 cleavage products adversely affects pre-implantation development and female fertility. PMID: 29158485
  2. Through an in-depth bioinformatic analysis of potential interactors, PGC7 was linked to critical cellular processes including translation, RNA processing, cell cycle, and regulation of heterochromatin structure. PMID: 28712289
  3. PRDM1 and STELLA, while overlapping in putative primordial germ cells, also co-localize in several other tissues PMID: 27696611
  4. The authors show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of maternal-to-zygotic transition. PMID: 28323615
  5. Data indicate STELLA's potential roles in building the fetal-placental connection. PMID: 28322735
  6. This study shows repression of the Dppa3 Locus in the absence of MAD2L2 embryonic stem cells. PMID: 28330620
  7. In-depth transcriptome analysis of this mESC state reveals Dppa3 as a direct downstream target of Tbx3.We show that Tbx3, a downstream target of Wnt signaling, fine tunes these divergent roles of Wnt signaling in mESCs PMID: 26095607
  8. Dppa3 has a critical role in generation of fully reprogrammed iPS cells and maintenance of Dlk1-Dio3 imprinting PMID: 25613421
  9. Stella protects maternal chromosomes from aberrant epigenetic modifications to ensure early embryogenesis. PMID: 25694116
  10. Chromocenter formation is impaired in Stella-null embryos. PMID: 26325466
  11. we propose that Dppa3 is involved in the Tet-mediated active demethylation process during reprogramming of primordial germ cells. PMID: 23595900
  12. PGC7 protects 5mC from Tet3-mediated conversion to 5hmC by binding to maternal chromatin containing dimethylated histone H3 lysine 9 (H3K9me2) in mice PMID: 22722204
  13. These Dppa3-MCM strains offer valuable opportunities to explore gene function in both loss-of-function and gain-of-function experiments at a variety of time points during germ cell development. PMID: 21525417
  14. DPPA3 is an essential factor for the production of functional oocytes through transcriptional repression and chromatin condensation. PMID: 22034526
  15. Data show that STELLA was found in both the nucleus and cytoplasm in a variety of cell types, both within and outside of the putative primordial germ cells (PGCs)trajectory. PMID: 22019303
  16. Data show that Stella is specifically expressed in preimplantation embryos and in the germline. PMID: 21786403
  17. embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage PMID: 14654002
  18. Dppa3 is expressed in germ cells and is a marker of cell pluripotency. PMID: 14684992
  19. a marker of pluripotency: Stella-positive ESCs were like the inner cell mass, Stella-negative cells were like the epiblast cells PMID: 18940731

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Subcellular Location
Nucleus. Cytoplasm. Note=Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono-laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In 3.5 dpc blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.
Tissue Specificity
Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial
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