Recombinant Mouse Diacylglycerol kinase alpha (Dgka)

Code CSB-YP006832MO
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Source Yeast
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Code CSB-EP006832MO
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Source E.coli
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Code CSB-EP006832MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006832MO
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Source Baculovirus
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Code CSB-MP006832MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dgka
Uniprot No.
Alternative Names
Dgka; Dagk1Diacylglycerol kinase alpha; DAG kinase alpha; EC 2.7.1.107; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
Species
Mus musculus (Mouse)
Expression Region
1-730
Target Protein Sequence
MAKEKGLISP EDFAQLQKYI EYSTKRVSDV LKVFDDGEMN RFCQGDAIGY LGFEQFMKMY LEMEEVPHHL CWALFWSFHT SQVAAEKTKS KANVICLSDV YCYFTLLEGG RPEDKLEFTF KLYDMDRNGI LDSTEVEKII LQMMRVAEYL DWDVSELRPI LQEMMREMDQ DGSGSVSLDE WVRAGATTVP LLVLLGMDVT MKDDGNHIWR PKRFTRLVYC NLCEQSISLG KQGLSCNFCK YIVHDHCAMK AQPCEVSTYA KSRKDIGVQS HLWVRGGCHS GRCDRCQKKI RTYHSLTGLH CVWCHLEIHD DCLQAVGPEC DCGLLRDHIL PPCSIYPSVL VSGQECKHKT TDDTSLCTPE AFRIEPVSNT HPLLVFINLK SGGKQGQSVL WKFQYILNPR QVFDLKDGPE PGLRFFKDVP QFRILVCGGD GTVGWVLETI DKANFATVPP VAVLPLGTGN DLARCLRWGR GYEGENLRKI LKDIELSKVV YLDRWFLEVI PQQNGEKSDP VPSQIINNYF SIGVDASIAH RFHLMREKYP EKFNSRMKNK LWYFEFATSE SIFSTCKKLE ESVTVEICGK LLDLSDLSLE GIAVLNIPST HGGSNLWGDT KRPHGDTCEI NQALGSAAKI ITDPDILKTC VPDMSDKRLE VVGIEGAIEM GQIYTRLKSA GHRLAKCSEI TFQTTKTLPM QIDGEPWMQA PCTIKITHKN QMPMLMGPPS NSYNFFGFWS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
Gene References into Functions
  1. Diacylglycerol kinases alpha and zeta are up-regulated in cancer in cancer, and contribute towards tumor immune evasion and T cells clonal anergy. (Review) PMID: 27697466
  2. Our studies demonstrate that PKD1/2 is a key regulator of MVB maturation and exosome secretion, and constitutes a mediator of the DGK alpha effect on MVB secretory traffic. PMID: 26045048
  3. Diacylglycerol kinase alpha establishes T cell polarity by shaping diacylglycerol accumulation at the immunological synapse. PMID: 25161317
  4. It plays a role in pathogenesis of various disorders in the central nervous system. PMID: 24599142
  5. Ovalbumin-induced airway inflammation was inhibited after DGKalpha DNA vaccine administation. PMID: 24228102
  6. Data indicate that combined deletion of dgkzeta and dgkalpha markedly enhances T-cell responses. PMID: 23576561
  7. The data strongly supported a role for FoxO proteins in promoting high diacylglycerol kinase alpha levels and indicate a mechanism by which diacylglycerol kinase alpha function is downregulated during productive T cell responses. PMID: 22890845
  8. results suggest that the globular form of adiponectin-induced generation of reactive oxygen species and nitric oxide involves diacylglycerol kinase alpha PMID: 21034360
  9. DAG kinases (DGKs) alpha and zeta, which terminate DAG-mediated signaling, synergistically inhibit TCR-induced mTOR activation by inhibiting the Ras-Mek1/2-Erk/12 pathway PMID: 21310925
  10. A role for DGK alpha in T cell activation is confirmed by the rapid DGK alpha translocation to the membrane fraction, together with the increase in enzyme activity that follows T cell activation in vivo. PMID: 12626538
  11. regulated by pi 3-kinase activation products PMID: 12832407
  12. Diacylglycerol kinase alpha is an anergy-associated molecule that negatively regulates Ras in anergic T cells. PMID: 17028589
  13. DGKs not only terminate diacylglycerol signaling but also initiate PA signaling in thymocytes to promote positive selection PMID: 18689679
  14. Diacylglycerol kinase alpha deficiency causes enhanced T cell activation and confers resistance to anergy induction in vitro and in vivo. PMID: 17028587

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Subcellular Location
Cytoplasm, cytosol.
Protein Families
Eukaryotic diacylglycerol kinase family
Database Links
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