Recombinant Mouse Dual specificity protein phosphatase CDC14B (Cdc14b)

Code CSB-YP740782MO
MSDS
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Source Yeast
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Code CSB-EP740782MO
MSDS
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Source E.coli
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Code CSB-EP740782MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP740782MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP740782MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cdc14b
Uniprot No.
Alternative Names
Cdc14bDual specificity protein phosphatase CDC14B; EC 3.1.3.16; EC 3.1.3.48; CDC14 cell division cycle 14 homolog B
Species
Mus musculus (Mouse)
Expression Region
1-485
Target Protein Sequence
MKRKSERRSA WATAPPCSRR SSSSSPGVKK SRSSTPQELH RLEQQDDLYL DITDRLCFAI LYSRPKSATN EHYFSIDNEL EYENFYADFG PLNLAMVYRY CCKINKKLKS ITMLRKKIIH FTGTDQRKQA NAAFLVGCYM VIYLGRTPED AYRTLIFGDT AYIPFRDAAY GSCSFYITLL DCFHAVKKAM QYGFFNFNSF NLDEYEHYEK AENGDFNWII PERFLAFCGP HSRSRLESGY HQHSPETYIP YFKNHNVTTI IRLNKRMYDA KRFTDAGFDH HDLFFPDGST PAESIVQEFL DICENVKGAI AVHCKAGLGR TGTLIGCYLM KHYRMTAAES IAWLRICRPG SVIGPQQQFL VMKQSSLWLE GDYFRQKLRG QENGPLREAF SKHLSDADDL SLNGLENQDN QEPEPYSDDD EVSGMTQGDR LRALKSRRQP KASAIPLTCP LAVLTSALCS VAIWWIVCDY ILPTLLFCLD GFRTQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.
Gene References into Functions
  1. Cdc14B knockout (Cdc14B(-/-)) mouse embryonic fibroblasts showed defects in repairing ionizing radiation-induced DNA double-strand breaks, which occurred only at late passages when Cdc14A levels were low. PMID: 26283732
  2. Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription PMID: 22355704
  3. Cdc14B has oncogenic activity in mammals and point to the Ras-MAP kinase pathway as a major effector pathway during oncogenic transformation. PMID: 21502810
  4. Cdc14b is required for efficient DNA damage repair. PMID: 21262768
  5. CDC14B is a negative regulator of the 1-to-2-cell transition and of zygotic genome activation in mouse embryogenesis. PMID: 19923902
  6. CDC14B is a negative regulator of meiotic resumption and may regulate meiosis I in mouse oocytes. PMID: 19129509

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Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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