Recombinant Mouse E3 ubiquitin-protein ligase CBL-B(Cblb) ,partial

Code CSB-YP667545MO
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Source Yeast
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Code CSB-EP667545MO
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Source E.coli
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Code CSB-EP667545MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP667545MO
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Source Baculovirus
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Code CSB-MP667545MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Cblb
Uniprot No. Q3TTA7
Alternative Names CblbE3 ubiquitin-protein ligase CBL-B; EC 2.3.2.27; Casitas B-lineage lymphoma proto-oncogene b; RING-type E3 ubiquitin transferase CBL-B; SH3-binding protein CBL-B; Signal transduction protein CBL-B
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Gene References into Functions
  1. The establishment of a new model of concurrent tissue-selective CBL/CBL-B deletion should allow a clear assessment of the tumor-intrinsic roles of CBL/CBL-B in non-myeloid malignancies and help test the potential for CBL/CBL-B inactivation in immunotherapy of tumors. PMID: 27276677
  2. GSK3 catalyzes two previously unreported phosphorylation events at Ser(476) and Ser(480) of Cbl-b. Constitutive activation of PKB in vivo results in a loss of tolerance that is mediated through the downregulation of Cbl-b. The PI3K-PKB-GSK-3 pathway is a novel regulatory axis that is important for controlling the decision between T cell activation and tolerance via Cbl-b. PMID: 29109121
  3. These studies reveal a novel, cell-autonomous requirement of CBL and CBL-B in epithelial stem cell maintenance during organ development and remodeling through modulation of mTOR signaling. PMID: 28100467
  4. CBLB directs polyubiquitination of dectin-1 and dectin-2, two key pattern-recognition receptors for sensing Candida albicans, and their downstream kinase SYK, thus inhibiting dectin-1- and dectin-2-mediated innate immune responses. PMID: 27428899
  5. CBLB controls proximal C-type lectin receptor signaling in macrophages and dendritic cells. CBLB associates with SYK and ubiquitinates SYK, dectin-1, and dectin-2 after fungal recognition. CBLB deficiency results in increased inflammasome activation, enhanced reactive oxygen species production, and increased fungal killing. PMID: 27428901
  6. study indicates that Cbl-b negatively regulates CLR-mediated antifungal innate immunity PMID: 27432944
  7. Fasudil, a clinically safe ROCK inhibitor, decreases disease burden in a Cbl/Cbl-b deficiency-driven murine model of myeloproliferative disorders. PMID: 26177294
  8. Mechanistically, NFATc1 induces Nur77 expression at late stage of osteoclast differentiation; in turn, Nur77 transcriptionally up-regulates E3 ubiquitin ligase Cbl-b, which triggers NFATc1 protein degradation. PMID: 26173181
  9. Silencing Cbl-b significantly enhanced T lymphocyte function and T lymphocyte cytotoxicity activity PMID: 25249301
  10. mechanisms have therapeutic implications for reducing beta-cell proliferation in insulinomas by inhibiting phospho-HLXB9 or its interaction with Nono and modulating the expression of its direct (Cblb) or indirect (c-Met) targets PMID: 26342078
  11. SHP-1 regulates Cbl-b-mediated T cell responses by controlling its tyrosine phosphorylation and ubiquitination PMID: 26416283
  12. Cbl-b(-/-) T cells demonstrate significant lymph node trafficking abnormalities. PMID: 25829233
  13. These results indicated that NF-kappaB downregulated Cbl-b by binding and suppressing Cbl-b promoter in T cell activation PMID: 25762784
  14. Cbl-b, together with Stub1, ubiquitinate Foxp3, and regulate tTreg development. PMID: 25560411
  15. Whereas Cbl-PI3K interaction regulates differentiation and survival, bone resorption is predominantly regulated by Cbl-b in osteoclasts. PMID: 24470255
  16. Cbl-b is the E3 ubiquitin ligase for Stat6. Cbl-b regulates Th9 in both Stat6-dependent and -independent mechanisms. PMID: 24508458
  17. The results demonstrate an essential and non-redundant role for Cbl-b in controlling TGFbetaR signaling by directly targeting SMAD7 for degradation during T cell responses in vitro and in vivo. PMID: 23709694
  18. Cbl-b, by promoting the ubiquitination and degradation of DDR2, functions as a negative regulator in the DDR2 signaling pathway. PMID: 24631539
  19. genetic deletion of the E3 ubiquitin ligase Cbl-b (casitas B-lineage lymphoma-b) or targeted inactivation of its E3 ligase activity licenses natural killer (NK) cells to spontaneously reject metastatic tumours PMID: 24553136
  20. Cbl-b plays only a limited role in the induction of antigen-specific T cell responses by murine bone marrow-derived dendritic cells in vitro and in vivo. PMID: 23762309
  21. study demonstrates that Cbl-b regulates the fate of inducible regulatory T cells via controlling the threshold for T cell activation PMID: 23749633
  22. Cbl-b inhibits T cell activation by suppressing Pten inactivation independently of its ubiquitin ligase activity. PMID: 22763434
  23. Data suggest that targeting Cbl-b might serve as a novel 'adjuvant approach', suitable to augment the effectiveness of established anti-cancer immunotherapies. PMID: 21383769
  24. c-Cbl as well as Cbl-b may play important roles in Hsp90 inhibitor-induced degradation of Flt3-ITD through the ubiquitin proteasome system PMID: 21768087
  25. Cbl-b is a critical factor in maintaining lung homeostasis upon environmental exposure to aeroallergens. PMID: 20738317
  26. Interplay between Cblb-dependent T cell anergy and other mechanisms prevents organ-specific islet-cell autoimmunity in transgenic mice. PMID: 21248249
  27. Selective genetic inactivation of Cbl-b E3 ligase activity phenocopies the T cell responses observed when total Cbl-b is ablated, resulting in T cell hyperactivation, spontaneous autoimmunity, and impaired induction of T cell anergy in vivo. PMID: 21248250
  28. The resilts of this study suggested that Cbl-b is an important regulatory factor for cytotoxic T-cell infiltration via RANTES production in macrophages. PMID: 21254087
  29. show that, in contrast to Cbl or Cbl-b single-deficient mice, concurrent loss of Cbl and Cbl-b in the HSC compartment leads to an early-onset lethal myeloproliferative disease in mice PMID: 20805496
  30. Data show that intrinsic regulation of peripheral T cells by CBL-B serves a uniquely critical role as a failsafe against clinical onset of autoimmune disease in AIRE deficiency. PMID: 20668237
  31. Data suggest that the regulatory abnormalities in Cbl-b(-/-) mice are related to defects in Teff, not Treg, function. PMID: 20624942
  32. gene deletion of both Cbl-b and Itch leads to augmented T cell activation and spontaneous autoimmunity PMID: 20637659
  33. Results indicate that Cbl-b controls Runx2 expression at the post-translational level. PMID: 20578243
  34. This study reveals that Foxo factors promote transcription of the Foxp3 gene in induced T reg cells, and thus provides new mechanistic insight into Foxo-mediated T cell regulation. PMID: 20439537
  35. Cbl-b plays a positive modulatory role in GPVI-dependent platelet signaling, which translates to an important regulatory role in hemostasis and thrombosis in vivo PMID: 20400514
  36. Cbl-b and itch are key regulators of peripheral T-cell tolerance [review] PMID: 20395198
  37. NFATc1 interacts endogenously with c-Src, c-Cbl, and Cbl-b in osteoclasts. PMID: 20037154
  38. novel E3 ubiquitin-protein ligase; role in regulation of immune response - review PMID: 11826757
  39. Cbl-b negatively regulates BCR signaling by targeting Syk for ubiquitination. PMID: 12771181
  40. both c-Cbl and Cbl-b can initiate a phosphatidylinositol 3-kinase/protein kinase B-independent signaling pathway critical to insulin-stimulated GLUT4 translocation PMID: 12842890
  41. Cbl-b and c-Cbl have divergent effects on Fc epsilon RI signal transduction and that Cbl-b, but not c-Cbl, functions as a negative regulator of Fc epsilon RI-induced degranulation. PMID: 15265912
  42. activated KIT in turn induces phosphorylation and activation of Cbl proteins PMID: 15315962
  43. Study provides a molecular framework for Cbl-b/Vav1-dependent and Cbl-b/Vav1-independent CD28 activation pathways essential for the organization of secondary lymphoid tissues and in vivo immunity to viral infection. PMID: 15661906
  44. E3 ubiquitin ligase Cbl-b plays an integral role in T cell TGF-beta signaling; its absence results in multifunctional TGF-beta-related defects that have important disease-related implications. PMID: 16424156
  45. cbl-b is a negative regulator of long-term memory, and its neuronal mechanism regulates synaptic transmission in the hippocampus PMID: 16549761
  46. results suggest that Cbl-b induces resistance of osteoblasts to IGF-I during denervation by increasing IRS-1 degradation and that Cbl-b-mediated modification of IGF-I signaling may contribute to decreased bone formation during denervation PMID: 16734387
  47. Cbl proteins play a critical role in establishing the MHC-dependent CD4(+) and CD8(+) T cell development programs. PMID: 17045823
  48. Cbl-b functions as a potent negative regulator of cytokines that promote allergic and inflammatory reactions. PMID: 17056522
  49. Ablation of Cbl-b can be an efficient strategy for eliciting immune responses against both inoculated and spontaneous tumors. PMID: 17364027
  50. identify Cbl-b as a key signaling molecule that controls spontaneous antitumor activity of cytotoxic T cells in different cancer models PMID: 17403934
  51. Cbl proteins control B cell-intrinsic checkpoint of immune tolerance, possibly through coordinating multiple B cell receptor-proximal signaling pathways during anergy induction PMID: 17493844
  52. Cbl-b is a negative regulator of macrophage infiltration and activation, and macrophage activation by Cbl-b deficiency contributes to the peripheral insulin resistance and glucose intolerance. PMID: 17601987
  53. Cblb regulates the TLR4-mediated acute inflammatory response that is induced by sepsis PMID: 17618294
  54. the ability of Cbl-b to function as a negative regulator of FcvarepsilonRI signalling that promotes inflammatory cytokine production is largely independent of the RING finger domain. PMID: 17868870
  55. Cbl-b attenuates CD40-mediated NF-kappaB and JNK activation, thereby suppressing B cell responses PMID: 17878343
  56. These data not only show that Cbl-b-imposed constraint on TCR signaling has differential effects on various facets of CD8 T cell response but also suggest that Cbl-b might mitigate tissue injury induced by the overproduction of IFN-gamma by CD8 T cells. PMID: 18025165
  57. in the murine lymphocytic choriomeningitis virus (LCMV) model, induction of the T-cell receptor signaling inhibitor molecule E3 ligase Cbl-b is critically involved in down-regulation of virus-specific T-cell response. PMID: 18199651
  58. Cbl-b associates with PKC-theta upon TCR stimulation and regulates TCR-induced PKC-theta activation via Vav-1, which couples PKC-theta to PI3-K and allows it to be phosphorylated. PMID: 18227156
  59. a novel role for Cbl-b in triggering the exit from cell cycle at the end of a CD4(+) T cell clonal expansion PMID: 18832689
  60. One of Cbl-b's unique functions in osteoclasts is to downregulate bone resorption. PMID: 19257814
  61. Results indicate that the Cbl-b-dependent destruction of IRS-1 is a critical dual mediator of both increased protein degradation and reduced protein synthesis observed in unloading-induced muscle atrophy. PMID: 19546233
  62. The biochemical program that ensures the induction of anergy uses several mechanisms, including the synthesis of ubiquitin E3 ligases such as Cbl-b (Casitas B-lineage lymphoma). PMID: 19549983
  63. a nonredundant antagonism between PKC-theta and Cbl-b that regulates T cell activation responses. PMID: 19549985
  64. The conserved four-helix bundle of c-Cbl's tyrosine kinase binding domain bound to beta-tubulin, and both c-Cbl and Cbl-b displaced HDAC6. PMID: 19641021
  65. Cbl-b deficiency may in part predispose individuals toward developing autoimmunity by increasing the number of anergic self-reactive T cells. PMID: 19801520

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Subcellular Location Cytoplasm
Database Links

KEGG: mmu:208650

STRING: 10090.ENSMUSP00000110115

UniGene: Mm.328206

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