Recombinant Mouse Ephrin-A3 (Efna3)

Code CSB-YP007462MO
MSDS
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Source Yeast
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Code CSB-EP007462MO
MSDS
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Source E.coli
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Code CSB-EP007462MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007462MO
MSDS
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Source Baculovirus
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Code CSB-MP007462MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Efna3
Uniprot No.
Alternative Names
Efna3; Epl3; Eplg3; Lerk3Ephrin-A3; EHK1 ligand; EHK1-L; EPH-related receptor tyrosine kinase ligand 3; LERK-3
Species
Mus musculus (Mouse)
Expression Region
23-206
Target Protein Sequence
QGPGGALG NRHAVYWNSS NQHLRREGYT VQVNVNDYLD IYCPHYNSSG PGGGAEQYVL YMVNLSGYRT CNASQGSKRW ECNRQHASHS PIKFSEKFQR YSAFSLGYEF HAGQEYYYIS TPTHNLHWKC LRMKVFVCCA STSHSGEKPV PTLPQFTMGP NVKINVLEDF EGENPQVPKL EKSISG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Gene References into Functions
  1. Findings suggest a putative novel mechanism for desipramine to modulate long-term potentiation through the regulation of the ephrinA3/EphA4 signaling pathway PMID: 26785076
  2. Ephrin-A3 suppresses Wnt signaling to control retinal stem cell potency. PMID: 23165658
  3. Downregulation of the EphA4 receptor via siRNA transfection reduced the repulsive effect of ephrin-A3, indicating that EphA4 mediates at least in part the repulsive effect of ephrin-A3. PMID: 20473036
  4. Data show that a number of Eph receptors and ephrins were expressed in hematopoietic stem cells. PMID: 20655977
  5. Ephrin-A3 is localized on astrocytic processes that envelop spines. Activation of EphA4 by ephrin-A3 caused spine retraction; inhibiting ephrin/EphA4 interactions distorted spine shape and organization in hippocampal slices. PMID: 12496762
  6. Organ of Corti and spiral ganglion showed strong expression of ephrin-A3, ephrin-B2 and ephrin-B3. In lateral wall, ephrin-A3 and ephrin-B2 were strongly expressed. Ephrin-A3 was strongly expressed in utricular and saccular sensory epithelia. PMID: 12684176
  7. Results demonstrate that neurons expressing different odorant receptors express different levels of ephrin-A3 and -A5 protein on their axons. PMID: 12914696
  8. ephrin-A3 not only accelerates anagen development but also increases the density of hair follicles. PMID: 18640011
  9. heparan sulfate modulates ephrin-A3/Eph signaling, suggesting a physiological role for heparan sulfate proteoglycans in the regulation of ephrin-A3-dependent biological processes PMID: 18715996
  10. Results suggest that the interaction between neuronal EphA4 and glial ephrin-A3 bidirectionally controls synapse morphology and glial glutamate transport, ultimately regulating hippocampal function. PMID: 19592509
  11. These results suggest that EphA4/ephrin-A3 signaling is a critical mechanism for astrocytes to regulate synaptic function and plasticity. PMID: 19734893

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Ephrin family
Tissue Specificity
Expressed in myogenic progenitor cells.
Database Links
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