Recombinant Mouse Epididymal secretory glutathione peroxidase (Gpx5)

Code CSB-YP009870MO
MSDS
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Source Yeast
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Code CSB-EP009870MO
MSDS
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Source E.coli
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Code CSB-EP009870MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009870MO
MSDS
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Source Baculovirus
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Code CSB-MP009870MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gpx5
Uniprot No.
Alternative Names
Gpx5Epididymal secretory glutathione peroxidase; EC 1.11.1.9; Epididymis-specific glutathione peroxidase-like protein; EGLP; Glutathione peroxidase 5; GPx-5; GSHPx-5; Major androgen-regulated protein; arMEP24
Species
Mus musculus (Mouse)
Expression Region
22-221
Target Protein Sequence
TPRPEKMKM DCYKDVKGTI YDYEALSLNG KEHIPFKQYA GKHVLFVNVA TYCGLTIQYP ELNALQEDLK PFGLVILGFP CNQFGKQEPG DNLEILPGLK YVRPGKGFLP NFQLFAKGDV NGENEQKIFT FLKRSCPHPS ETVVMSKHTF WEPIKVHDIR WNFEKFLVGP DGIPVMRWFH QAPVSTVKSD IMAYLSHFKT I
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids.
Gene References into Functions
  1. enzymatic epididymal salvage response is sufficient to maintain full fertility of double GPx4 and GPx5 KO males whatever their age, crossed with young WT female mice PMID: 22719900
  2. GPX5 is found in the caput & cauda epididymis lumen in 3 locations: as a soluble protein in the caput epididymal fluid, weakly bound to caput sperm membranes, or associated to lipid-containing structures causing a protective effect against proteolysis. PMID: 12211066
  3. GPX5 gene is expressed in a temporally regulated manner during mouse embryonic development. PMID: 18577359
  4. results reveal that expression of Nm23-M5 plays a critical role in spermiogenesis by increasing the cellular levels of GPX-5 to eliminate reactive oxygen species PMID: 19303412
  5. GPX5 is a potent antioxidant scavenger in the luminal compartment of the mouse cauda epididymidis that protects spermatozoa from oxidative injuries. PMID: 19546506

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Subcellular Location
Secreted.
Protein Families
Glutathione peroxidase family
Tissue Specificity
Epididymis.
Database Links
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